BRAVO ANAYA, Lourdes Monica M; PETITDEMANGE, Rosine; ROSSELIN, Marie; IBARBOURE, Emmanuel; GARBAY, Bertrand; GARANGER, Elisabeth; DEMING, Timothy; LECOMMANDOUX, Sebastien

doi:10.1021/acs.biomac.0c00374

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BRAVO ANAYA, Lourdes Monica M
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences

PETITDEMANGE, Rosine
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences

ROSSELIN, Marie
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences

Langue

Article de revue

Ce document a été publié dans

Biomacromolecules. 2020

American Chemical Society

Résumé en anglais

Selective lectin binding and sorting was achieved using thermosensitive glycoconjugates derived from recombinant elastin-like polypeptides (ELPs) in simple centrifugation-precipitation assays. A recombinant ELP, (VPGXG)40, containing periodically spaced methionine residues was used to enable chemoselective post-synthetic modification via thioether alkylation using alkyne functional epoxide derivatives. The resulting sulfonium groups were selectively demethylated to give alkyne functionalized homocysteine residues, which were then reacted with azido-functionalized monosaccharides to obtain ELP glycoconjugates with periodic saccharide functionality. These modifications were also found to allow modulation of ELP temperature dependent water solubility. The multivalent ELP glycoconjugates were evaluated for specific recognition, binding and separation of the lectin Ricinus communis agglutinin (RCA120) from a complex protein mixture. RCA120 and ELP glycoconjugate interactions were evaluated using laser scanning confocal microscopy and dynamic light scattering. Due to the thermoresponsive nature of the ELP glycoconjugates, it was found that heating a mixture of galactose-functionalized ELP and RCA120 in complex media selectively yielded a phase separated pellet of ELP-RCA120 complexes. Based on these results, ELP glycoconjugates show promise as designer biopolymers for selective protein binding and sorting.< Réduire

Mots clés en anglais

click chemistry

lectins

chemoselective post-modifications

Elastin-like polypeptides (ELPs)

saccharides

glycoconjugates

URI

https://oskar-bordeaux.fr/handle/20.500.12278/19671

DOI

http://dx.doi.org/10.1021/acs.biomac.0c00374

Project ANR

Développement de squelettes polypeptidiques recombinants pour la synthèse de glycoconjugués multivalents parfaitement définis - ANR-15-CE07-0002

Origine

Importé de hal

Unités de recherche

Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629