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Biomarker responses of Eisenia andrei to a polymetallic gradient near a lead mining site in North Tunisia

hal.structure.identifierLaboratory of Biochemistry and Environmental Toxicology
dc.contributor.authorBOUGHATTAS, Iteb
hal.structure.identifierLaboratory of Soil Sciences
dc.contributor.authorHATTAB, Sabrine
hal.structure.identifierLaboratory of Biochemistry and Environmental Toxicology
dc.contributor.authorBOUSSETTA, Hamadi
hal.structure.identifierInteractions Sol Plante Atmosphère [UMR ISPA]
dc.contributor.authorSAPPIN-DIDIER, Valerie
hal.structure.identifierDepartment of Sciences and Technological Innovation (DiSIT)
dc.contributor.authorVIARENGO, Aldo
hal.structure.identifierLaboratory of Biochemistry and Environmental Toxicology
hal.structure.identifierDepartment of Sciences and Technological Innovation (DiSIT)
dc.contributor.authorBANNI, Mohamed
hal.structure.identifierDepartment of Sciences and Technological Innovation (DiSIT)
dc.contributor.authorSFORZINI, Susanna
dc.date.accessioned2024-04-08T12:09:49Z
dc.date.available2024-04-08T12:09:49Z
dc.date.issued2016
dc.identifier.issn0269-7491
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/196593
dc.description.abstractEnEisenia andrei earthworms were exposed for 7 and 14 days to six samples of soil taken from around an abandoned lead (Pb) mine and characterized by different levels of metal contamination (S6-S1, this latter being the most contaminated soil). The organisms were analyzed for metal bioaccumulation and for biological parameters as biomarkers of stress (lysosomal membrane stability; lipofuscin lysosomal content; lysosomal/cytoplasmic volume ratio) and genotoxicity (Micronucleus frequency). Chemical analysis showed the loads of Pb, Cd, Zn, and Cu in the worms following exposure. Among the stress biomarkers, lysosomal membrane stability was significantly affected in the coelomocytes of the earthworms exposed already 7 days to different contaminated soils. Organisms exposed for 14 days to S1 showed in the cells of the chloragogenous tissue, a particularly relevant increase in lipofuscin, a biomarker of oxidative stress, and an increase in the lysosome/cytoplasm volume ratio, indicating stressful condition at the tissue level. Moreover, in the same conditions, a decrease in total body weight was observed. At the longer exposure time, the coelomocytes of worms exposed to S1, S2, and S3 (soils with higher metal concentrations) showed a significant increase in micronuclei (MNi) frequency. Expressions of the P21 and topoisomerase genes, which are involved in DNA repair, showed significant up-regulation in the cells of worms exposed to S1, S2, S3, S4 and to a less extend S6. This may indicate that the worms were only able to successfully reduce the level of DNA damage in S4 and S5 if considering MN frequency data. The biomarker data was integrated by the Earthworm Expert System, allowing an objective interpretation of the complex biological data and clearly defining the areas in which the presence of chemicals is toxic for the edaphic organisms.
dc.language.isoen
dc.publisherElsevier
dc.subjectplomb
dc.subjectpollution métallique
dc.subject.enEisenia andrei
dc.subject.enmining sites
dc.subject.enDNA alterations
dc.subject.enLMS
dc.subject.engene expression
dc.subject.enlead
dc.title.enBiomarker responses of Eisenia andrei to a polymetallic gradient near a lead mining site in North Tunisia
dc.typeArticle de revue
dc.identifier.doi10.1016/j.envpol.2016.07.033
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalEnvironmental Pollution
bordeaux.page530-541
bordeaux.volume218
bordeaux.hal.laboratoriesInteractions Soil Plant Atmosphere (ISPA) - UMR 1391*
bordeaux.institutionBordeaux Sciences Agro
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-01604934
hal.version1
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01604934v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Environmental%20Pollution&rft.date=2016&rft.volume=218&rft.spage=530-541&rft.epage=530-541&rft.eissn=0269-7491&rft.issn=0269-7491&rft.au=BOUGHATTAS,%20Iteb&HATTAB,%20Sabrine&BOUSSETTA,%20Hamadi&SAPPIN-DIDIER,%20Valerie&VIARENGO,%20Aldo&rft.genre=article


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