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Characterizing the Core-Shell Architecture of Block Copolymer Nanoparticles with Electron Microscopy: A Multi-Technique Approach

dc.rights.licenseopen
hal.structure.identifierInstitut de Science des Matériaux de Mulhouse [IS2M]
dc.contributor.authorTKACHENKO, Vitalii
hal.structure.identifierInstitut de Science des Matériaux de Mulhouse [IS2M]
dc.contributor.authorVIDAL, Loïc
hal.structure.identifierInstitut de Science des Matériaux de Mulhouse [IS2M]
dc.contributor.authorJOSIEN, Ludovic
hal.structure.identifierInstitut Charles Sadron [ICS]
dc.contributor.authorSCHMUTZ, Marc
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
dc.contributor.authorPOLY, Julien
hal.structure.identifierInstitut de Science des Matériaux de Mulhouse [IS2M]
dc.contributor.authorCHEMTOB, Abraham
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2020
dc.identifier.issn2073-4360
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/19625
dc.description.abstractEnElectron microscopy has proved to be a major tool to study the structure of self-assembled amphiphilic block copolymer particles. These specimens, like supramolecular biological structures, are problematic for electron microscopy because of their poor capacity to scatter electrons and their susceptibility to radiation damage and dehydration. Sub-50 nm core-shell spherical particles made up of poly(hydroxyethyl acrylate)-b-poly(styrene) are prepared via polymerization-induced self-assembly (PISA). For their morphological characterization, we discuss the advantages, limitations, and artefacts of TEM with or without staining, cryo-TEM, and SEM. A number of technical points are addressed such as precisely shaping of particle boundaries, resolving the particle shell, differentiating particle core and shell, and the effect of sample drying and staining. TEM without staining and cryo-TEM largely evaluate the core diameter. Negative staining TEM is more efficient than positive staining TEM to preserve native structure and to visualize the entire particle volume. However, no technique allows for a satisfactory imaging of both core and shell regions. The presence of long protruding chains is manifested by patched structure in cryo-TEM and a significant edge effect in SEM. This manuscript provides a basis for polymer chemists to develop their own specimen preparations and to tackle the interpretation of challenging systems.
dc.language.isoen
dc.publisherMDPI
dc.subject.encore-shell
dc.subject.enTEM
dc.subject.encryo-TEM
dc.subject.enSEM
dc.subject.enpositive staining
dc.subject.ennegative staining
dc.subject.enPISA
dc.subject.ennanoparticles
dc.title.enCharacterizing the Core-Shell Architecture of Block Copolymer Nanoparticles with Electron Microscopy: A Multi-Technique Approach
dc.typeArticle de revue
dc.identifier.doi10.3390/polym12081656
dc.subject.halChimie/Polymères
bordeaux.journalPolymers
bordeaux.page1656
bordeaux.volume12
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue8
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-02908459
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02908459v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Polymers&rft.date=2020&rft.volume=12&rft.issue=8&rft.spage=1656&rft.epage=1656&rft.eissn=2073-4360&rft.issn=2073-4360&rft.au=TKACHENKO,%20Vitalii&VIDAL,%20Lo%C3%AFc&JOSIEN,%20Ludovic&SCHMUTZ,%20Marc&POLY,%20Julien&rft.genre=article


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