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Contrasting effects of nicotianamine synthase knockdown on zinc and nickel tolerance and accumulation in the zinc/cadmium hyperaccumulator Arabidopsis halleri

hal.structure.identifierInteractions Sol Plante Atmosphère [UMR ISPA]
dc.contributor.authorCORNU, Jean-Yves
dc.contributor.authorDEINLEIN, Ulrich
dc.contributor.authorHORETH, Stephan
dc.contributor.authorBRAUN, Manuel
dc.contributor.authorSCHMIDT, Holger
dc.contributor.authorWEBER, Michael
hal.structure.identifierUniversity of Copenhagen = Københavns Universitet [UCPH]
dc.contributor.authorPERSSON, Daniel P.
hal.structure.identifierUniversity of Copenhagen = Københavns Universitet [UCPH]
dc.contributor.authorHUSTED, Soren
hal.structure.identifierUniversity of Copenhagen = Københavns Universitet [UCPH]
dc.contributor.authorSCHJOERRING, Jan K.
dc.contributor.authorCLEMENS, Stephan
dc.date.accessioned2024-04-08T12:02:19Z
dc.date.available2024-04-08T12:02:19Z
dc.date.issued2015
dc.identifier.issn0028-646X
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/196196
dc.description.abstractEnElevated nicotianamine synthesis in roots of Arabidopsis halleri has been established as a zinc (Zn) hyperaccumulation factor. The main objective of this study was to elucidate the mechanism of nicotianamine-dependent root-to-shoot translocation of metals. Metal tolerance and accumulation in wild-type (WT) and AhNAS2-RNA interference (RNAi) plants were analysed. Xylem exudates were subjected to speciation analysis and metabolite profiling. Suppression of root nicotianamine synthesis had no effect on Zn and cadmium (Cd) tolerance but rendered plants nickel (Ni)-hypersensitive. It also led to a reduction of Zn root-to-shoot translocation, yet had the opposite effect on Ni mobility, even though both metals form coordination complexes of similar stability with nicotianamine. Xylem Zn concentrations were positively, yet nonstoichiometrically, correlated with nicotianamine concentrations. Two fractions containing Zn coordination complexes were detected in WT xylem. One of them was strongly reduced in AhNAS2-suppressed plants and coeluted with Zn-67-labelled organic acid complexes. Organic acid concentrations were not responsive to nicotianamine concentrations and sufficiently high to account for complexing the coordinated Zn. We propose a key role for nicotianamine in controlling the efficiency of Zn xylem loading and thereby the formation of Zn coordination complexes with organic acids, which are the main Zn ligands in the xylem but are not rate-limiting for Zn translocation.
dc.language.isoen
dc.publisherWiley
dc.subject.enArabidopsis halleri
dc.subject.enmetal hyperaccumulation
dc.subject.enmetal tolerance
dc.subject.ennicotianamine (NA)
dc.subject.enorganic acids
dc.subject.enspeciation analysis
dc.subject.enxylem transport
dc.title.enContrasting effects of nicotianamine synthase knockdown on zinc and nickel tolerance and accumulation in the zinc/cadmium hyperaccumulator Arabidopsis halleri
dc.typeArticle de revue
dc.identifier.doi10.1111/nph.13237
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalNew Phytologist
bordeaux.page738-750
bordeaux.volume206
bordeaux.hal.laboratoriesInteractions Soil Plant Atmosphere (ISPA) - UMR 1391*
bordeaux.issue2
bordeaux.institutionBordeaux Sciences Agro
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-02636206
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02636206v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=New%20Phytologist&rft.date=2015&rft.volume=206&rft.issue=2&rft.spage=738-750&rft.epage=738-750&rft.eissn=0028-646X&rft.issn=0028-646X&rft.au=CORNU,%20Jean-Yves&DEINLEIN,%20Ulrich&HORETH,%20Stephan&BRAUN,%20Manuel&SCHMIDT,%20Holger&rft.genre=article


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