Hydrogel embedded quantum dot-transcription factor sensor for quantitative progesterone detection
dc.rights.license | open | |
hal.structure.identifier | Boston University [Boston] [BU] | |
dc.contributor.author | CHEN, Mingfu | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
dc.contributor.author | GRAZON, Chloé | |
hal.structure.identifier | Boston University [Boston] [BU] | |
dc.contributor.author | SENSHARMA, Prerana | |
hal.structure.identifier | Boston University [Boston] [BU] | |
dc.contributor.author | NGUYEN, Thuy | |
hal.structure.identifier | Boston University [Boston] [BU] | |
dc.contributor.author | FENG, Yunpeng | |
hal.structure.identifier | Boston University [Boston] [BU] | |
dc.contributor.author | CHERN, Margaret | |
hal.structure.identifier | Boston University [Boston] [BU] | |
dc.contributor.author | BAER, R. | |
hal.structure.identifier | Boston University [Boston] [BU] | |
dc.contributor.author | VARONGCHAYAKUL, Nitinun | |
hal.structure.identifier | Boston University [Boston] [BU] | |
dc.contributor.author | COOK, Katherine | |
hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
dc.contributor.author | LECOMMANDOUX, Sebastien | |
hal.structure.identifier | Boston University [Boston] [BU] | |
dc.contributor.author | KLAPPERICH, Catherine | |
hal.structure.identifier | Boston University [Boston] [BU] | |
dc.contributor.author | GALAGAN, James | |
hal.structure.identifier | Boston University [Boston] [BU] | |
dc.contributor.author | DENNIS, Allison | |
hal.structure.identifier | Boston University [Boston] [BU] | |
dc.contributor.author | GRINSTAFF, Mark | |
dc.date.accessioned | 2020 | |
dc.date.available | 2020 | |
dc.date.issued | 2020 | |
dc.identifier.issn | 1944-8244 | |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/19568 | |
dc.description.abstractEn | Immobilization of biosensors in or on a functional material is critical for subsequent device development and translation to wearable technology. Here we present the development and assessment of an immobilized quantum dot - transcription factor - nucleic acid complex for progesterone detection as a first step toward such device integration. The sensor is composed of a polyhistidine-tagged transcription factor linked to a quantum dot and a fluorophore-modified cognate DNA, and embedded within a hydrogel as an immobilization matrix. The hydrogel is optically transparent, soft, and flexible as well as traps the quantum dot - transcription factor DNA assembly but allows free passage of the analyte, progesterone. Upon progesterone exposure, DNA dissociates from the quantum dot - transcription factor DNA assembly resulting in an attenuated ratiometric fluorescent output via Förster resonance energy transfer. The sensor performs in a dose-dependent manner with a limit of detection of 55 nM. Repeat analyte measurements are also similarly successful. Our approach combines a systematically characterized hydrogel as an immobilization matrix and a transcription factor - DNA binding as a recognition/ transduction element, offering a promising framework for future biosensor devices based upon allosteric transcription factor. | |
dc.language.iso | en | |
dc.publisher | Washington, D.C. : American Chemical Society | |
dc.subject.en | biosensing | |
dc.subject.en | quantum dots | |
dc.subject.en | transcription factor | |
dc.subject.en | hydrogel | |
dc.subject.en | Förster resonance energy transfer | |
dc.title.en | Hydrogel embedded quantum dot-transcription factor sensor for quantitative progesterone detection | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1021/acsami.0c13489 | |
dc.subject.hal | Chimie/Polymères | |
dc.subject.hal | Physique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft] | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM] | |
dc.subject.hal | Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Endocrinologie et métabolisme | |
dc.description.sponsorshipEurope | A Wearable Sensor for Hormones Based on a Native Microbial Sensing | |
bordeaux.journal | ACS Applied Materials & Interfaces | |
bordeaux.page | doi 10.1021/acsami.0c13489 | |
bordeaux.volume | 12 | |
bordeaux.hal.laboratories | Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629 | * |
bordeaux.institution | Bordeaux INP | |
bordeaux.institution | Université de Bordeaux | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-02939691 | |
hal.version | 1 | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-02939691v1 | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=ACS%20Applied%20Materials%20&%20Interfaces&rft.date=2020&rft.volume=12&rft.spage=doi%2010.1021/acsami.0c13489&rft.epage=doi%2010.1021/acsami.0c13489&rft.eissn=1944-8244&rft.issn=1944-8244&rft.au=CHEN,%20Mingfu&GRAZON,%20Chlo%C3%A9&SENSHARMA,%20Prerana&NGUYEN,%20Thuy&FENG,%20Yunpeng&rft.genre=article |
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