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Hydrogel embedded quantum dot-transcription factor sensor for quantitative progesterone detection

dc.rights.licenseopen
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorCHEN, Mingfu
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorGRAZON, Chloé
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorSENSHARMA, Prerana
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorNGUYEN, Thuy
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorFENG, Yunpeng
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorCHERN, Margaret
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorBAER, R.
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorVARONGCHAYAKUL, Nitinun
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorCOOK, Katherine
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorLECOMMANDOUX, Sebastien
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorKLAPPERICH, Catherine
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorGALAGAN, James
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorDENNIS, Allison
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorGRINSTAFF, Mark
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2020
dc.identifier.issn1944-8244
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/19568
dc.description.abstractEnImmobilization of biosensors in or on a functional material is critical for subsequent device development and translation to wearable technology. Here we present the development and assessment of an immobilized quantum dot - transcription factor - nucleic acid complex for progesterone detection as a first step toward such device integration. The sensor is composed of a polyhistidine-tagged transcription factor linked to a quantum dot and a fluorophore-modified cognate DNA, and embedded within a hydrogel as an immobilization matrix. The hydrogel is optically transparent, soft, and flexible as well as traps the quantum dot - transcription factor DNA assembly but allows free passage of the analyte, progesterone. Upon progesterone exposure, DNA dissociates from the quantum dot - transcription factor DNA assembly resulting in an attenuated ratiometric fluorescent output via Förster resonance energy transfer. The sensor performs in a dose-dependent manner with a limit of detection of 55 nM. Repeat analyte measurements are also similarly successful. Our approach combines a systematically characterized hydrogel as an immobilization matrix and a transcription factor - DNA binding as a recognition/ transduction element, offering a promising framework for future biosensor devices based upon allosteric transcription factor.
dc.language.isoen
dc.publisherWashington, D.C. : American Chemical Society
dc.subject.enbiosensing
dc.subject.enquantum dots
dc.subject.entranscription factor
dc.subject.enhydrogel
dc.subject.enFörster resonance energy transfer
dc.title.enHydrogel embedded quantum dot-transcription factor sensor for quantitative progesterone detection
dc.typeArticle de revue
dc.identifier.doi10.1021/acsami.0c13489
dc.subject.halChimie/Polymères
dc.subject.halPhysique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft]
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologie/Endocrinologie et métabolisme
dc.description.sponsorshipEuropeA Wearable Sensor for Hormones Based on a Native Microbial Sensing
bordeaux.journalACS Applied Materials & Interfaces
bordeaux.pagedoi 10.1021/acsami.0c13489
bordeaux.volume12
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-02939691
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02939691v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=ACS%20Applied%20Materials%20&%20Interfaces&rft.date=2020&rft.volume=12&rft.spage=doi%2010.1021/acsami.0c13489&rft.epage=doi%2010.1021/acsami.0c13489&rft.eissn=1944-8244&rft.issn=1944-8244&rft.au=CHEN,%20Mingfu&GRAZON,%20Chlo%C3%A9&SENSHARMA,%20Prerana&NGUYEN,%20Thuy&FENG,%20Yunpeng&rft.genre=article


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