Modelling central metabolic fluxes by constraint-based optimization reveals metabolic reprogramming of developing Solanum lycopersicum (tomato) fruit.
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | COLOMBIÉ, Sophie | |
| hal.structure.identifier | Institut de Mathématiques de Bordeaux [IMB] | |
| dc.contributor.author | NAZARET, Christine | |
| hal.structure.identifier | UNIROUEN - UFR Santé [UNIROUEN UFR Santé] | |
| dc.contributor.author | BÉNARD, Camille | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | BIAIS, Benoît | |
| dc.contributor.author | MENGIN, Virginie | |
| dc.contributor.author | SOLÉ, Marion | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | FOUILLEN, Laetitia | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | DIEUAIDE-NOUBHANI, Martine | |
| hal.structure.identifier | Physiopathologie mitochondriale | |
| dc.contributor.author | MAZAT, Jean-Pierre | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | BEAUVOIT, Bertrand | |
| hal.structure.identifier | 1074 Institut de Biologie Végétale Moléculaire : actions communes | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | GIBON, Yves | |
| dc.date.accessioned | 2024-04-04T03:11:30Z | |
| dc.date.available | 2024-04-04T03:11:30Z | |
| dc.date.issued | 2015-01 | |
| dc.identifier.issn | 0960-7412 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/193782 | |
| dc.description.abstractEn | Modelling of metabolic networks is a powerful tool to analyse the behaviour of developing plant organs, including fruits. Guided by our current understanding of heterotrophic metabolism of plant cells, a medium-scale stoichiometric model, including the balance of co-factors and energy, was constructed in order to describe metabolic shifts that occur through the nine sequential stages of Solanum lycopersicum (tomato) fruit development. The measured concentrations of the main biomass components and the accumulated metabolites in the pericarp, determined at each stage, were fitted in order to calculate, by derivation, the corresponding external fluxes. They were used as constraints to solve the model by minimizing the internal fluxes. The distribution of the calculated fluxes of central metabolism were then analysed and compared with known metabolic behaviours. For instance, the partition of the main metabolic pathways (glycolysis, pentose phosphate pathway, etc.) was relevant throughout fruit development. We also predicted a valid import of carbon and nitrogen by the fruit, as well as a consistent CO2 release. Interestingly, the energetic balance indicates that excess ATP is dissipated just before the onset of ripening, supporting the concept of the climacteric crisis. Finally, the apparent contradiction between calculated fluxes with low values compared with measured enzyme capacities suggest a complex reprogramming of the metabolic machinery during fruit development. With a powerful set of experimental data and an accurate definition of the metabolic system, this work provides important insight into the metabolic and physiological requirements of the developing tomato fruits. | |
| dc.description.sponsorship | Centre français de phénomique végétale - ANR-11-INBS-0012 | |
| dc.language.iso | en | |
| dc.publisher | Wiley | |
| dc.subject.en | central metabolism | |
| dc.subject.en | constraint-based analysis | |
| dc.subject.en | flux balance analysis | |
| dc.subject.en | fruit metabolism | |
| dc.subject.en | modelling | |
| dc.subject.en | systems biology | |
| dc.title.en | Modelling central metabolic fluxes by constraint-based optimization reveals metabolic reprogramming of developing Solanum lycopersicum (tomato) fruit. | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1111/tpj.12685 | |
| dc.subject.hal | Sciences du Vivant [q-bio] | |
| dc.subject.hal | Mathématiques [math] | |
| bordeaux.journal | Plant Journal | |
| bordeaux.page | 24-39 | |
| bordeaux.volume | 81 | |
| bordeaux.hal.laboratories | Institut de Mathématiques de Bordeaux (IMB) - UMR 5251 | * |
| bordeaux.issue | 1 | |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.institution | Bordeaux INP | |
| bordeaux.institution | CNRS | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-01445680 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-01445680v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plant%20Journal&rft.date=2015-01&rft.volume=81&rft.issue=1&rft.spage=24-39&rft.epage=24-39&rft.eissn=0960-7412&rft.issn=0960-7412&rft.au=COLOMBI%C3%89,%20Sophie&NAZARET,%20Christine&B%C3%89NARD,%20Camille&BIAIS,%20Beno%C3%AEt&MENGIN,%20Virginie&rft.genre=article |
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