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Correction for Photobleaching in Dynamic Fluorescence Microscopy: Application in the Assessment of Pharmacokinetic Parameters in Ultrasound-Mediated Drug Delivery

hal.structure.identifierUniversity Medical Center [Utrecht] [UMCU]
dc.contributor.authorDERIEPPE, Marc
hal.structure.identifierUniversity Medical Center [Utrecht] [UMCU]
dc.contributor.authorBOS, Clemens
hal.structure.identifierUniversity Medical Center [Utrecht] [UMCU]
dc.contributor.authorDE GREEF, M.
hal.structure.identifierUniversity Medical Center [Utrecht] [UMCU]
dc.contributor.authorMOONEN, Chrit
hal.structure.identifierUniversity Medical Center [Utrecht] [UMCU]
hal.structure.identifierInstitut de Mathématiques de Bordeaux [IMB]
dc.contributor.authorDENIS DE SENNEVILLE, Baudouin
dc.date.accessioned2024-04-04T03:09:06Z
dc.date.available2024-04-04T03:09:06Z
dc.date.issued2015-12-01
dc.identifier.issn0031-9155
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/193582
dc.description.abstractEnPurpose. We have previously demonstrated the feasibility of monitoring ultrasound-mediated uptake of a hydrophilic model drug in real time with dynamic confocal fluorescence microscopy. In this study, we evaluate and correct the impact of photobleaching to improve the accuracy of pharmacokinetic parameter estimates.Procedures. To model photobleaching of the fluorescent model drug SYTOX Green, a photobleaching process was added to the current two-compartment model describing cell uptake. After collection of the uptake profile, a second acquisition was performed when SYTOX Green was equilibrated, to evaluate the photobleaching rate experimentally.Results. Photobleaching rates up to 5.0 10-3 s-1 were measured when applying power densities up to 0.2 W.cm-2. By applying the three-compartment model, the model drug uptake rate of 6.0 10-3 s-1 was measured independent of the applied laser power.Conclusions. The impact of photobleaching on uptake rate estimates measured by dynamic fluorescence microscopy was evaluated. Subsequent compensation improved the accuracy of pharmacokinetic parameter estimates in the cell population subjected to sonopermeabilization.
dc.language.isoen
dc.publisherIOP Publishing
dc.title.enCorrection for Photobleaching in Dynamic Fluorescence Microscopy: Application in the Assessment of Pharmacokinetic Parameters in Ultrasound-Mediated Drug Delivery
dc.typeArticle de revue
dc.subject.halSciences de l'ingénieur [physics]/Traitement du signal et de l'image
bordeaux.journalPhysics in Medicine and Biology
bordeaux.page588
bordeaux.volume61
bordeaux.hal.laboratoriesInstitut de Mathématiques de Bordeaux (IMB) - UMR 5251*
bordeaux.issue2
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-01259337
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01259337v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Physics%20in%20Medicine%20and%20Biology&rft.date=2015-12-01&rft.volume=61&rft.issue=2&rft.spage=588&rft.epage=588&rft.eissn=0031-9155&rft.issn=0031-9155&rft.au=DERIEPPE,%20Marc&BOS,%20Clemens&DE%20GREEF,%20M.&MOONEN,%20Chrit&DENIS%20DE%20SENNEVILLE,%20Baudouin&rft.genre=article


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