hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
dc.contributor.author | BELOUAH, Isma | |
hal.structure.identifier | Institut de Mathématiques de Bordeaux [IMB] | |
dc.contributor.author | NAZARET, Christine | |
hal.structure.identifier | Interactions Plantes Pathogènes [IPP] | |
dc.contributor.author | PÉTRIACQ, Pierre | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | PRIGENT, Sylvain | |
hal.structure.identifier | UNIROUEN - UFR Santé [UNIROUEN UFR Santé] | |
dc.contributor.author | BÉNARD, Camille | |
hal.structure.identifier | Max Planck Institute of Molecular Plant Physiology [MPI-MP] | |
dc.contributor.author | MENGIN, Virginie | |
hal.structure.identifier | Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) [GQE-Le Moulon] | |
dc.contributor.author | BLEIN-NICOLAS, Melisande | |
dc.contributor.author | DENTON, Alisandra | |
hal.structure.identifier | Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) [GQE-Le Moulon] | |
dc.contributor.author | BALLIAU, Thierry | |
dc.contributor.author | AUGÉ, Ségolène | |
hal.structure.identifier | Laboratoire de Génétique Cellulaire [LGC] | |
dc.contributor.author | BOUCHEZ, Olivier | |
hal.structure.identifier | Physiopathologie mitochondriale | |
dc.contributor.author | MAZAT, Jean-Pierre | |
hal.structure.identifier | Max Planck Institute of Molecular Plant Physiology [MPI-MP] | |
dc.contributor.author | STITT, Mark | |
hal.structure.identifier | BioEcon Sci Ctr, Inst Bot & Mol Genet | |
dc.contributor.author | USADEL, Björn | |
hal.structure.identifier | Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) [GQE-Le Moulon] | |
dc.contributor.author | ZIVY, Michel | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | BEAUVOIT, Bertrand | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | GIBON, Yves | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | COLOMBIE, Sophie | |
dc.date.accessioned | 2024-04-04T02:59:27Z | |
dc.date.available | 2024-04-04T02:59:27Z | |
dc.date.issued | 2019-06-28 | |
dc.identifier.issn | 0032-0889 | |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/192743 | |
dc.description.abstractEn | Protein synthesis and degradation are essential processes that regulate cell status. Because labeling in bulky organs, such as fruits, is difficult, we developed a modeling approach to study protein turnover at the global scale in developing tomato (Solanum lycopersicum) fruit. Quantitative data were collected for transcripts and proteins during fruit development. Clustering analysis showed smaller changes in protein abundance compared to mRNA abundance. Furthermore, protein and transcript abundance were poorly correlated, and the coefficient of correlation decreased during fruit development and ripening, with transcript levels decreasing more than protein levels. A mathematical model with one ordinary differential equation was used to estimate translation (kt) and degradation (kd) rate constants for almost 2,400 detected transcript-protein pairs and was satisfactorily fitted for over a thousand pairs. The model predicted median values of about 2 min for the translation of a protein, and a protein lifetime of approximately 11 days. The constants were validated and inspected for biological relevance. Proteins involved in protein synthesis had higher kt and kd values, indicating that the protein machinery is particularly flexible. Our model also predicts that protein concentration is more strongly affected by the rate of translation than that of degradation. | |
dc.description.sponsorship | Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010 | |
dc.language.iso | en | |
dc.publisher | Oxford University Press ; American Society of Plant Biologists | |
dc.title.en | Modeling Protein Destiny in Developing Fruit | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1104/pp.19.00086 | |
dc.subject.hal | Sciences du Vivant [q-bio] | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN] | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale | |
dc.subject.hal | Sciences du Vivant [q-bio]/Bio-Informatique, Biologie Systémique [q-bio.QM] | |
bordeaux.journal | Plant Physiology | |
bordeaux.page | 1709-1724 | |
bordeaux.volume | 180 | |
bordeaux.hal.laboratories | Institut de Mathématiques de Bordeaux (IMB) - UMR 5251 | * |
bordeaux.issue | 3 | |
bordeaux.institution | Université de Bordeaux | |
bordeaux.institution | Bordeaux INP | |
bordeaux.institution | CNRS | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-02327302 | |
hal.version | 1 | |
hal.popular | non | |
hal.audience | Internationale | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-02327302v1 | |
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