Targeting Ultrastructural Events at the Graft Interface of Arabidopsis thaliana by A Correlative Light Electron Microscopy Approach
| dc.rights.license | open | en_US |
| hal.structure.identifier | Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV] | |
| dc.contributor.author | CHAMBAUD, Clément | |
| hal.structure.identifier | Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV] | |
| dc.contributor.author | COOKSON, Sarah J.
IDREF: 22161009X | |
| hal.structure.identifier | Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV] | |
| dc.contributor.author | OLLAT, Nathalie
IDREF: 126740062 | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | BERNARD, Amélie
IDREF: 157438902 | |
| hal.structure.identifier | Bordeaux Imaging Center [BIC] | |
| dc.contributor.author | BROCARD, Lysiane | |
| dc.date.accessioned | 2024-01-31T14:07:04Z | |
| dc.date.available | 2024-01-31T14:07:04Z | |
| dc.date.issued | 2023-01-20 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/187708 | |
| dc.description.abstractEn | Combining two different plants together through grafting is one of the oldest horticultural techniques. In order to survive, both partners must communicate via the formation of de novo connections between the scion and the rootstock. Despite the importance of grafting, the ultrastructural processes occurring at the graft interface remain elusive due to the difficulty of locating the exact interface at the ultrastructural level. To date, only studies with interfamily grafts showing enough ultrastructural differences were able to reliably localize the grafting interface at the ultrastructural level under electron microscopy. Thanks to the implementation of correlative light electron microscopy (CLEM) approaches where the grafted partners were tagged with fluorescent proteins of different colors, the graft interface was successfully and reliably targeted. Here, we describe a protocol for CLEM for the model plant Arabidopsis thaliana, which unambiguously targets the graft interface at the ultrastructural level. Moreover, this protocol is compatible with immunolocalization and electron tomography acquisition to achieve a three-dimensional view of the ultrastructural events of interest in plant tissues. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | Arabidopsis Thaliana | |
| dc.subject.en | Correlative Microscopy | |
| dc.subject.en | Electron Microscopy | |
| dc.subject.en | Electron Tomography | |
| dc.subject.en | Plant Grafting | |
| dc.title.en | Targeting Ultrastructural Events at the Graft Interface of Arabidopsis thaliana by A Correlative Light Electron Microscopy Approach | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.21769/BioProtoc.4590 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale | en_US |
| bordeaux.journal | Bio-protocol | en_US |
| bordeaux.volume | 13 | en_US |
| bordeaux.hal.laboratories | Ecophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287 | en_US |
| bordeaux.issue | 2 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | Bordeaux Sciences Agro | en_US |
| bordeaux.institution | INRAE | en_US |
| bordeaux.institution | CNRS | |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | false | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Bio-protocol&rft.date=2023-01-20&rft.volume=13&rft.issue=2&rft.au=CHAMBAUD,%20Cl%C3%A9ment&COOKSON,%20Sarah%20J.&OLLAT,%20Nathalie&BERNARD,%20Am%C3%A9lie&BROCARD,%20Lysiane&rft.genre=article |
