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Ephrin-B1 regulates the adult diastolic function through a late postnatal maturation of cardiomyocyte surface crests

dc.rights.licenseopenen_US
hal.structure.identifierInstitut des Maladies Métaboliques et Casdiovasculaires [UPS/Inserm U1297 - I2MC]
dc.contributor.authorKARSENTY, Clement
hal.structure.identifierInstitut des Maladies Métaboliques et Casdiovasculaires [UPS/Inserm U1297 - I2MC]
dc.contributor.authorGUILBEAU-FRUGIER, Celine
hal.structure.identifierUniversity of Virginia
dc.contributor.authorGENET, Gaël
hal.structure.identifierInstitut des Maladies Métaboliques et Casdiovasculaires [UPS/Inserm U1297 - I2MC]
dc.contributor.authorSEGUELAS, Marie-Helene
hal.structure.identifierBiologie des maladies cardiovasculaires = Biology of Cardiovascular Diseases
dc.contributor.authorALZIEU, Philippe
hal.structure.identifierPhysiologie & médecine expérimentale du Cœur et des Muscles [U 1046] [PhyMedExp]
dc.contributor.authorCAZORLA, Olivier
hal.structure.identifierInstitut des Maladies Métaboliques et Casdiovasculaires [UPS/Inserm U1297 - I2MC]
dc.contributor.authorMONTAGNER, Alexandra
hal.structure.identifierInstitut de Génétique et Développement de Rennes [IGDR]
dc.contributor.authorBLUM, Yuna
dc.contributor.authorDUBROCA, Caroline
dc.contributor.authorMAUPOINT, Julile
dc.contributor.authorTRAMUNT, Blandine
dc.contributor.authorCAUQUIL, Marie
dc.contributor.authorSULPICE, Thierry
hal.structure.identifierPhysiologie & médecine expérimentale du Cœur et des Muscles [U 1046] [PhyMedExp]
dc.contributor.authorRICHARD, Sylvain
dc.contributor.authorARCUCCI, Silvia
dc.contributor.authorFLORES-FLORES, Remy
dc.contributor.authorPATALUCH, Nicolas
dc.contributor.authorMONTORIOL, Romain
hal.structure.identifierPhysiologie & médecine expérimentale du Cœur et des Muscles [U 1046] [PhyMedExp]
hal.structure.identifierCentre Hospitalier Régional Universitaire [Montpellier] [CHRU Montpellier]
dc.contributor.authorSICARD, Pierre
dc.contributor.authorDENEY, Antoine
dc.contributor.authorCOUFFINHAL, Thierry
dc.contributor.authorSENARD, Jean-Michel
hal.structure.identifierInstitut des Maladies Métaboliques et Casdiovasculaires [UPS/Inserm U1297 - I2MC]
hal.structure.identifierCentre Hospitalier Universitaire de Toulouse [CHU Toulouse]
dc.contributor.authorGALÉS, Celine
dc.date.accessioned2024-01-16T14:51:57Z
dc.date.available2024-01-16T14:51:57Z
dc.date.issued2023-01-17
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/187276
dc.description.abstractEnThe rod-shaped adult cardiomyocyte (CM) harbors a unique architecture of its lateral surface with periodic crests, relying on the presence of subsarcolemmal mitochondria (SSM) with unknown role. Here, we investigated the development and functional role of CM crests during the postnatal period. We found in rodents that CM crest maturation occurs late between postnatal day 20 (P20) and P60 through both SSM biogenesis, swelling and crest-crest lateral interactions between adjacent CM, promoting tissue compaction. At the functional level, we showed that the P20-P60 period is dedicated to the improvement of relaxation. Interestingly, crest maturation specifically contributes to an atypical CM hypertrophy of its short axis, without myofibril addition, but relying on CM lateral stretching. Mechanistically, using constitutive and conditional CM-specific knock-out mice, we identified ephrin-B1, a lateral membrane stabilizer, as a molecular determinant of P20-P60 crest maturation, governing both the CM lateral stretch and the diastolic function, thus highly suggesting a link between crest maturity and diastole. Remarkably, while young adult CM-specific Efnb1 KO mice essentially exhibit an impairment of the ventricular diastole with preserved ejection fraction and exercise intolerance, they progressively switch toward systolic heart failure with 100% KO mice dying after 13 months, indicative of a critical role of CM-ephrin-B1 in the adult heart function. This study highlights the molecular determinants and the biological implication of a new late P20-P60 postnatal developmental stage of the heart in rodents during which, in part, ephrin-B1 specifically regulates the maturation of the CM surface crests and of the diastolic function.
dc.language.isoENen_US
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.encardiac hypertrophy
dc.subject.encardiac postnatal devleopment
dc.subject.encardiomyocyte architecture
dc.subject.encell biology
dc.subject.endevelopmental biology
dc.subject.endiatolic function
dc.subject.enephrin-B1
dc.subject.enmouse
dc.subject.enrat
dc.subject.ensubsarcolemmal mitochondria
dc.subject.encardiac hypertrophy
dc.title.enEphrin-B1 regulates the adult diastolic function through a late postnatal maturation of cardiomyocyte surface crests
dc.typeArticle de revueen_US
dc.identifier.doi10.7554/eLife.80904en_US
dc.subject.halSciences du Vivant [q-bio]en_US
bordeaux.journaleLifeen_US
bordeaux.pagee80904en_US
bordeaux.volume12en_US
bordeaux.hal.laboratoriesBiologie des maladies cardiovasculaires (BMC) - UMR 1034en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINSERMen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-03944430
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=eLife&rft.date=2023-01-17&rft.volume=12&rft.spage=e80904&rft.epage=e80904&rft.au=KARSENTY,%20Clement&GUILBEAU-FRUGIER,%20Celine&GENET,%20Ga%C3%ABl&SEGUELAS,%20Marie-Helene&ALZIEU,%20Philippe&rft.genre=article


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