Comparison of methods for detection of plasmid-mediated and chromosomally encoded colistin resistance in Enterobacteriaceae.
| dc.rights.license | open | en_US |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | JAYOL, Aurelie | |
| hal.structure.identifier | Université de Fribourg = University of Fribourg [UNIFR] | |
| dc.contributor.author | NORDMANN, Patrice | |
| hal.structure.identifier | Laboratoire de bactériologie | |
| hal.structure.identifier | CHU Bordeaux | |
| dc.contributor.author | LEHOURS, Philippe | |
| hal.structure.identifier | Institut National de la Santé et de la Recherche Médicale [INSERM] | |
| hal.structure.identifier | Université de Fribourg = University of Fribourg [UNIFR] | |
| hal.structure.identifier | Infection, Anti-microbiens, Modélisation, Evolution [IAME (UMR_S_1137 / U1137)] | |
| dc.contributor.author | POIREL, Laurent | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | DUBOIS, Veronique | |
| dc.date.accessioned | 2023-12-04T12:49:18Z | |
| dc.date.available | 2023-12-04T12:49:18Z | |
| dc.date.issued | 2018-02-01 | |
| dc.identifier.issn | 1469-0691 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/186336 | |
| dc.description.abstractEn | Because of the emergence of plasmid-mediated (mcr-1 and mcr-2 genes) and chromosomally encoded colistin resistance, reliable methods for detecting colistin resistance/susceptibility in routine laboratories are required. We evaluated the respective performances of the BD Phoenix automated system, the newly developed Rapid Polymyxin NP test and the broth microdilution (BMD) reference method to detect colistin resistance in Enterobacteriaceae, and particularly those producing mcr-1 and mcr-2. Colistin susceptibility of 123 enterobacterial clinical isolates (40 colistin-susceptible and 83 colistin-resistant isolates) was tested with the BD Phoenix automated system, the Rapid Polymyxin NP test and the BMD method. Molecular mechanisms responsible for plasmid-mediated and chromosomally encoded colistin resistance mechanisms were investigated by PCR and sequencing. Considering BMD as a reference method, the BD Phoenix system failed to detect ten colistin-resistant isolates (one Escherichia coli, one Klebsiella pneumoniae, seven Enterobacter species and one Salmonella enterica). The Rapid Polymyxin NP test failed to detect the same single E. coli isolate. Those two latter methods detected the 16 E. coli, K. pneumoniae and S. enterica isolates producing the plasmid-encoded mcr-1 and mcr-2. The BD Phoenix system and the Rapid Polymyxin NP test are reliable techniques for detecting plasmid-mediated mcr-1 and mcr-2-related colistin resistance. However, a high rate of false susceptibility was observed with the BD Phoenix system, indicating that susceptibility results obtained with that system should be confirmed by BMD method. By contrast, the Rapid Polymyxin NP test showed a good agreement with the BMD method, and results were obtained rapidly (within 2 hours). The BMD method should be performed if minimum inhibitory concentration values are needed. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | Anti-Bacterial Agents | |
| dc.subject.en | Chromosomes | |
| dc.subject.en | Bacterial | |
| dc.subject.en | Colistin | |
| dc.subject.en | Drug Resistance | |
| dc.subject.en | Bacterial | |
| dc.subject.en | Enterobacteriaceae | |
| dc.subject.en | Humans | |
| dc.subject.en | Microbial Sensitivity Tests | |
| dc.subject.en | Plasmids | |
| dc.title.en | Comparison of methods for detection of plasmid-mediated and chromosomally encoded colistin resistance in Enterobacteriaceae. | |
| dc.title.alternative | Clin Microbiol Infect | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1016/j.cmi.2017.06.002 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Médecine humaine et pathologie | en_US |
| dc.identifier.pubmed | 28606644 | en_US |
| bordeaux.journal | Clinical Microbiology and Infection | en_US |
| bordeaux.page | 175-179 | en_US |
| bordeaux.volume | 24 | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.issue | 2 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | pubmed | |
| hal.identifier | hal-04321010 | |
| hal.version | 1 | |
| hal.date.transferred | 2023-12-04T12:49:19Z | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | true | |
| workflow.import.source | pubmed | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Clinical%20Microbiology%20and%20Infection&rft.date=2018-02-01&rft.volume=24&rft.issue=2&rft.spage=175-179&rft.epage=175-179&rft.eissn=1469-0691&rft.issn=1469-0691&rft.au=JAYOL,%20Aurelie&NORDMANN,%20Patrice&LEHOURS,%20Philippe&POIREL,%20Laurent&DUBOIS,%20Veronique&rft.genre=article |
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