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Vers une gestion des maladies basée sur le microbiote : analyse du microbiote de la vigne dans des parcelles présentant des niveaux contrastés d'infection par le mildiou

hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
hal.structure.identifierAmélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]
hal.structure.identifierDiversité, Adaptation et Amélioration de la Vigne [AGAP] [DAAV]
dc.contributor.authorFOURNIER, Paola
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorJASWA, Aarti
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorPELLAN, Lucile
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorVALLANCE, Jessica
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorCHANCEREL, Emilie
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorBONNARD, Olivier
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorMASNEUF-POMARÈDE, Isabelle
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorMARTINS, Guilherme
hal.structure.identifierSanté des Écosystèmes Viticoles Économes en intraNts [UMT SEVEN]
dc.contributor.authorRAYNAL, Marc
hal.structure.identifierSanté des Écosystèmes Viticoles Économes en intraNts [UMT SEVEN]
dc.contributor.authorDEBORD, Christian
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLABARTHE, Simon
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorDELIERE, Laurent
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorDELMOTTE, François
hal.structure.identifierAmélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]
hal.structure.identifierDiversité, Adaptation et Amélioration de la Vigne [AGAP] [DAAV]
dc.contributor.authorTHIS, Patrice
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorVACHER, Corinne
dc.date.issued2023-07-05
dc.date.conference2023-07-17
dc.description.abstractEnContext and purpose of the study: Vineyards harbor a myriad of microorganisms that interact with each other and with the grapevines. Some microorganisms are plant pathogens, such as the oomycete Plasmopara viticola that causes grapevine downy mildew. Others, such as plant growth promoting bacteria and disease biocontrol agents, have a positive influence on vine health. The present study aims to (1) investigate whether vine-based culture media increase the cultivability of the grapevine microbiota, in comparison to standard culture media and (2) identify and isolate bacterial taxa naturally present in grapevine leaves and significantly more abundant in plots showing low susceptibility to downy mildew.Material and methods: Seven pairs of vineyard plots differing significantly in downy mildew symptoms frequency and intensity were selected based on a long-term epidemiological survey conducted in France by the Institut Français de la Vigne et du Vin (IFV). In each plot, we sampled young leaves before the first fungicide treatments. Leaves wereshredded and washed in a buffer to isolate and cultivate foliar bacteria, yeasts and filamentous fungi. We assessed the abundance (CFU/ml) of cultivable bacterial and fungal cells for several culture media, including a vine-based culture medium. Bacterial isolates were identified using MALDI-TOF MS.Results: Yeasts, filamentous fungi, and bacteria were significantly less abundant on the vine-based culture medium than on the other media. Out of 965 total bacterial isolates analyzed, 597 were identified at least to the genus level by MALDI-TOF MS, and of these, 23% to the species level. Two bacterial genera, Gordonia and Rahnella, wereisolated exclusively on the vine-based culture medium. On the other hand, 10 genera, Rahnella, Paracoccus, Acinetobacter, Nocardia, Achromobacter, Peribacillus, Solibacillu, Xanthomona, Budvicia and Paenibacillus were isolated exclusively from samples collected from plots with low infection levels.
dc.description.sponsorshipCultivating the grapevine without pesticides : towards agroecological wine-producing socio-ecosystems - ANR-20-PCPA-0010
dc.description.sponsorshipBiosurveillance Next-Gen des changements dans la structure et le fonctionnement des écosystèmes - ANR-17-CE32-0011
dc.language.isoen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/
dc.subject.enGrapevine
dc.subject.endowny mildew
dc.subject.enmicrobial biodiversity
dc.subject.enculturomics
dc.subject.enphyllosphere
dc.subject.enbiocontrol
dc.titleVers une gestion des maladies basée sur le microbiote : analyse du microbiote de la vigne dans des parcelles présentant des niveaux contrastés d'infection par le mildiou
dc.title.enTowards microbiota-based disease management: analysis of grapevine microbiota in plots with contrasted levels of downy mildew infection
dc.typeAutre communication scientifique (congrès sans actes - poster - séminaire...)
dc.subject.halSciences du Vivant [q-bio]/Sciences agricoles
dc.subject.halSciences du Vivant [q-bio]/Biodiversité
dc.subject.halSciences du Vivant [q-bio]/Biodiversité/Systématique, phylogénie et taxonomie
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Ecologie, Environnement/Interactions entre organismes
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie
dc.subject.halSciences du Vivant [q-bio]/Sciences agricoles/Sciences et techniques de l'agriculture
bordeaux.conference.title22nd GiESCO CONGRESS (GiESCO 2023)
bordeaux.countryUS
bordeaux.conference.cityIthaca, New York
bordeaux.peerReviewedoui
hal.identifierhal-04180613
hal.version1
hal.invitednon
hal.proceedingsoui
hal.conference.organizerGroup of international Experts for Cooperation on Vitivinicultural Systems (GIESCO))
hal.conference.end2023-07-20
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04180613v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.title=Vers%20une%20gestion%20des%20maladies%20bas%C3%A9e%20sur%20le%20microbiote%20:%20analyse%20du%20microbiote%20de%20la%20vigne%20dans%20des%20parcelles%20pr%C3%A9sentant%20&rft.atitle=Vers%20une%20gestion%20des%20maladies%20bas%C3%A9e%20sur%20le%20microbiote%20:%20analyse%20du%20microbiote%20de%20la%20vigne%20dans%20des%20parcelles%20pr%C3%A9sentant%2&rft.date=2023-07-05&rft.au=FOURNIER,%20Paola&JASWA,%20Aarti&PELLAN,%20Lucile&VALLANCE,%20Jessica&CHANCEREL,%20Emilie&rft.genre=conference


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