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dc.contributor.authorLIU, Liwang
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorSIMON, Marina
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorMUGGIOLU, Giovanna
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorVILOTTE, Florent
dc.contributor.authorANTOINE, Mikael
dc.contributor.authorCARON, Jerôme
dc.contributor.authorKANTOR, Guy
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorBARBERET, Philippe
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorSEZNEC, Hervé
dc.contributor.authorAUDOIN, Bertrand
dc.date.accessioned2023-11-20T16:48:19Z
dc.date.available2023-11-20T16:48:19Z
dc.date.issued2022
dc.identifier.issn2213-5979
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/185059
dc.description.abstractEnHow DNA damage and repair processes affect the biomechanical properties of the nucleus interior remains unknown. Here, an opto-acoustic microscope based on time-domain Brillouin spectroscopy (TDBS) was used to investigate the induced regulation of intra-nuclear mechanics. With this ultrafast pump-probe technique, coherent acoustic phonons were tracked along their propagation in the intra-nucleus nanostructure and the complex stiffness moduli and thicknesses were measured with an optical resolution. Osteosarcoma cells were exposed to methyl methanesulfonate (MMS) and the presence of DNA damage was tested using immunodetection targeted against damage signaling proteins. TDBS revealed that the intra-nuclear storage modulus decreased significantly upon exposure to MMS, as a result of the chromatin decondensation and reorganization that favors molecular diffusion within the organelle. When the damaging agent was removed and cells incubated for 2 h in the buffer solution before fixation the intra-nuclear reorganization led to an inverse evolution of the storage modulus, the nucleus stiffened. The same tendency was measured when DNA double-strand breaks were caused by cell exposure to ionizing radiation. TDBS microscopy also revealed changes in acoustic dissipation, another mechanical probe of the intra-nucleus organization at the nano-scale, and changes in nucleus thickness during exposure to MMS and after recovery.
dc.description.sponsorshipEchographie opto-acoustique de cellules individuelles ex vivo
dc.language.isoen
dc.publisherElsevier
dc.title.enChanges in intra-nuclear mechanics in response to DNA damaging agents revealed by time-domain Brillouin micro-spectroscopy
dc.typeArticle de revue
dc.identifier.doi10.1016/j.pacs.2022.100385
dc.subject.halPhysique [physics]
bordeaux.journalPhotoacoustics
bordeaux.page100385
bordeaux.volume27
bordeaux.hal.laboratoriesCentre d'Études Nucléaires de Bordeaux Gradignan (CENBG)*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-03757798
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03757798v1
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