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Single α-particle irradiation permits real-time visualization of RNF8 accumulation at DNA damaged sites

hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorMUGGIOLU, Giovanna
hal.structure.identifierLaboratoire Capteurs Diamant (CEA, LIST) [LCD (CEA, LIST)]
dc.contributor.authorPOMORSKI, Michal
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorCLAVERIE, Gérard
hal.structure.identifierLaboratoire Capteurs Diamant (CEA, LIST) [LCD (CEA, LIST)]
dc.contributor.authorBERTHET, Guillaume
hal.structure.identifierLaboratoire Capteurs Diamant (CEA, LIST) [LCD (CEA, LIST)]
dc.contributor.authorMER-CALFATI, Christine
hal.structure.identifierLaboratoire Capteurs Diamant (CEA, LIST) [LCD (CEA, LIST)]
dc.contributor.authorSAADA, Samuel
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorDEVÈS, Guillaume
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorSIMON, Marina
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorSEZNEC, Hervé
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorBARBERET, Philippe
dc.date.accessioned2023-11-20T16:39:28Z
dc.date.available2023-11-20T16:39:28Z
dc.date.issued2017
dc.identifier.issn2045-2322
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/184957
dc.description.abstractEnAs well as being a significant source of environmental radiation exposure, α-particles are increasingly considered for use in targeted radiation therapy. A better understanding of α-particle induced damage at the DNA scale can be achieved by following their tracks in real-time in targeted living cells. Focused α-particle microbeams can facilitate this but, due to their low energy (up to a few MeV) and limited range, α-particles detection, delivery, and follow-up observations of radiation-induced damage remain difficult. In this study, we developed a thin Boron-doped Nano-Crystalline Diamond membrane that allows reliable single α-particles detection and single cell irradiation with negligible beam scattering. The radiation-induced responses of single 3 MeV α-particles delivered with focused microbeam are visualized in situ over thirty minutes after irradiation by the accumulation of the GFP-tagged RNF8 protein at DNA damaged sites.
dc.language.isoen
dc.publisherNature Publishing Group
dc.subject.eninstrumentation
dc.subject.ennuclear instrumentation
dc.subject.enionizing radiation
dc.subject.enradioactivity
dc.subject.enalpha-rays
dc.subject.enradiotherapy
dc.subject.enalpha particles
dc.subject.enradiation-induced damage
dc.subject.endiamond
dc.subject.enboron doping
dc.subject.ennano-crystalline diamond
dc.title.enSingle α-particle irradiation permits real-time visualization of RNF8 accumulation at DNA damaged sites
dc.typeArticle de revue
dc.identifier.doi10.1038/srep41764
dc.subject.halPhysique [physics]/Physique [physics]/Instrumentations et Détecteurs [physics.ins-det]
dc.subject.halPhysique [physics]/Physique [physics]/Physique Médicale [physics.med-ph]
bordeaux.journalScientific Reports
bordeaux.page41764
bordeaux.volume7
bordeaux.hal.laboratoriesCentre d'Études Nucléaires de Bordeaux Gradignan (CENBG)*
bordeaux.issue1
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-04146052
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04146052v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific%20Reports&rft.date=2017&rft.volume=7&rft.issue=1&rft.spage=41764&rft.epage=41764&rft.eissn=2045-2322&rft.issn=2045-2322&rft.au=MUGGIOLU,%20Giovanna&POMORSKI,%20Michal&CLAVERIE,%20G%C3%A9rard&BERTHET,%20Guillaume&MER-CALFATI,%20Christine&rft.genre=article


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