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In Vivo Labelling of Adenovirus DNA Identifies Chromatin Anchoring and Biphasic Genome Replication

dc.rights.licenseopenen_US
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorKOMATSU, Tetsuro
dc.contributor.authorQUENTIN-FROIGNANT, Charlotte
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorCARLON-ANDRES, Irene
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorLAGADEC, Floriane
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorRAYNE, Fabienne
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorRAGUES, Jessica
dc.contributor.authorKEHLENBACH, Ralph H
dc.contributor.authorZHANG, Wenli
hal.structure.identifierUniversität Witten/Herdecke
dc.contributor.authorEHRHARDT, Anja
dc.contributor.authorBYSTRICKY, Kerstin
dc.contributor.authorMORIN, Renaud
dc.contributor.authorLAGARDE, Jean-Michel
dc.contributor.authorGALLARDO, Franck
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorWODRICH, Harald
dc.date.accessioned2023-11-13T13:02:28Z
dc.date.available2023-11-13T13:02:28Z
dc.date.issued2018-09-15
dc.identifier.issn1098-5514en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/184733
dc.description.abstractEnAdenoviruses are DNA viruses with a lytic infection cycle. Following the fate of incoming as well as recently replicated genomes during infections is a challenge. In this study, we used the ANCHOR3 technology based on a bacterial partitioning system to establish a versatile imaging system for adenoviral genomes. The system allows the visualization of both individual incoming and newly replicated genomes in real time in living cells. We demonstrate that incoming adenoviral genomes are attached to condensed cellular chromatin during mitosis, facilitating the equal distribution of viral genomes in daughter cells after cell division. We show that the formation of replication centers occurs in conjunction with genome replication and determine replication rates. Visualization of adenoviral DNA revealed that adenoviruses exhibit two kinetically distinct phases of genome replication. Low-level replication occurred during early replication, while high-level replication was associated with late replication phases. The transition between these phases occurred concomitantly with morphological changes of viral replication compartments and with the appearance of virus-induced postreplication (ViPR) bodies, identified by the nucleolar protein Mybbp1A. Taken together, our real-time genome imaging system revealed hitherto uncharacterized features of adenoviral genomes The system is able to identify novel spatiotemporal aspects of the adenovirus life cycle and is potentially transferable to other viral systems with a double-stranded DNA phase. Viruses must deliver their genomes to host cells to ensure replication and propagation. Characterizing the fate of viral genomes is crucial to understand the viral life cycle and the fate of virus-derived vector tools. Here, we integrated the ANCHOR3 system, an DNA-tagging technology, into the adenoviral genome for real-time genome detection. ANCHOR3 tagging permitted the visualization of incoming genomes at the onset of infection and of replicated genomes at late phases of infection. Using this system, we show viral genome attachment to condensed host chromosomes during mitosis, identifying this mechanism as a mode of cell-to-cell transfer. We characterize the spatiotemporal organization of adenovirus replication and identify two kinetically distinct phases of viral genome replication. The ANCHOR3 system is the first technique that allows the continuous visualization of adenoviral genomes during the entire virus life cycle, opening the way for further in-depth study.
dc.language.isoENen_US
dc.subject.enAdenoviridae
dc.subject.enCell Line
dc.subject.enChromatin
dc.subject.enDNA
dc.subject.enViral
dc.subject.enDNA-Binding Proteins
dc.subject.enGenome
dc.subject.enViral
dc.subject.enHEK293 Cells
dc.subject.enHumans
dc.subject.enKinetics
dc.subject.enLife Cycle Stages
dc.subject.enNuclear Proteins
dc.subject.enNucleocytoplasmic Transport Proteins
dc.subject.enRNA-Binding Proteins
dc.subject.enStaining and Labeling
dc.subject.enTranscription Factors
dc.subject.enVirus Attachment
dc.subject.enVirus Replication
dc.title.enIn Vivo Labelling of Adenovirus DNA Identifies Chromatin Anchoring and Biphasic Genome Replication
dc.title.alternativeJ Virolen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1128/JVI.00795-18en_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologieen_US
dc.identifier.pubmed29997215en_US
bordeaux.journalJournal of Virologyen_US
bordeaux.volume92en_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue18en_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
hal.identifierhal-04282310
hal.version1
hal.date.transferred2023-11-13T13:02:30Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcepubmed
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Virology&rft.date=2018-09-15&rft.volume=92&rft.issue=18&rft.eissn=1098-5514&rft.issn=1098-5514&rft.au=KOMATSU,%20Tetsuro&QUENTIN-FROIGNANT,%20Charlotte&CARLON-ANDRES,%20Irene&LAGADEC,%20Floriane&RAYNE,%20Fabienne&rft.genre=article


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