Dual and Opposite Effects of hRAD51 Chemical Modulation on HIV-1 Integration.
| dc.rights.license | open | en_US |
| dc.contributor.author | THIERRY, Sylvain | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | BENLEULMI, Mohamed Salah | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | SINZELLE, Ludivine | |
| dc.contributor.author | THIERRY, Eloise | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | CALMELS, Christina | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | CHAIGNEPAIN, Stephane | |
| hal.structure.identifier | Groupe d’Etudes des Substances Végétales à Activité Biologique [GESVAB ] | |
| dc.contributor.author | WAFFO-TEGUO, Pierre | |
| hal.structure.identifier | Groupe d’Etudes des Substances Végétales à Activité Biologique [GESVAB ] | |
| dc.contributor.author | MERILLON, Jean-Michel | |
| dc.contributor.author | BUDKE, Brian | |
| hal.structure.identifier | INSERM U1035, Université de Bordeaux, UFR Sciences de la Vie et de la Santé, 33000 Bordeaux | |
| dc.contributor.author | PASQUET, Jean-Max | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | LITVAK, Simon | |
| dc.contributor.author | CIUFFI, Angela | |
| dc.contributor.author | SUNG, Patrick | |
| dc.contributor.author | CONNELL, Philip | |
| dc.contributor.author | HAUBER, Ilona | |
| dc.contributor.author | HAUBER, Joachim | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | ANDREOLA, Marie-Line | |
| dc.contributor.author | DELELIS, Olivier | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | PARISSI, Vincent | |
| dc.date.accessioned | 2023-11-13T11:57:29Z | |
| dc.date.available | 2023-11-13T11:57:29Z | |
| dc.date.issued | 2015-06-18 | |
| dc.identifier.issn | 1879-1301 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/184728 | |
| dc.description.abstractEn | The cellular DNA repair hRAD51 protein has been shown to restrict HIV-1 integration both in vitro and in vivo. To investigate its regulatory functions, we performed a pharmacological analysis of the retroviral integration modulation by hRAD51. We found that, in vitro, chemical activation of hRAD51 stimulates its integration inhibitory properties, whereas inhibition of hRAD51 decreases the integration restriction, indicating that the modulation of HIV-1 integration depends on the hRAD51 recombinase activity. Cellular analyses demonstrated that cells exhibiting high hRAD51 levels prior to de novo infection are more resistant to integration. On the other hand, when hRAD51 was activated during integration, cells were more permissive. Altogether, these data establish the functional link between hRAD51 activity and HIV-1 integration. Our results highlight the multiple and opposite effects of the recombinase during integration and provide new insights into the cellular regulation of HIV-1 replication. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | 4 | |
| dc.subject.en | 4'-Diisothiocyanostilbene-2 | |
| dc.subject.en | 2'-Disulfonic Acid | |
| dc.subject.en | Aptamers | |
| dc.subject.en | Nucleotide | |
| dc.subject.en | Benzamides | |
| dc.subject.en | DNA | |
| dc.subject.en | DNA Repair | |
| dc.subject.en | Gene Expression | |
| dc.subject.en | HEK293 Cells | |
| dc.subject.en | HIV-1 | |
| dc.subject.en | Humans | |
| dc.subject.en | Morpholines | |
| dc.subject.en | Protein Binding | |
| dc.subject.en | Pyrroles | |
| dc.subject.en | Rad51 Recombinase | |
| dc.subject.en | Stilbenes | |
| dc.subject.en | Sulfonamides | |
| dc.subject.en | Virus Internalization | |
| dc.subject.en | Virus Replication | |
| dc.title.en | Dual and Opposite Effects of hRAD51 Chemical Modulation on HIV-1 Integration. | |
| dc.title.alternative | Chem Biol | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1016/j.chembiol.2015.04.020 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie | en_US |
| dc.identifier.pubmed | 26051216 | en_US |
| bordeaux.journal | Journal of Chemical Biology | en_US |
| bordeaux.page | 712-23 | en_US |
| bordeaux.volume | 22 | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.issue | 6 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | pubmed | |
| hal.identifier | hal-04282149 | |
| hal.version | 1 | |
| hal.date.transferred | 2023-11-13T11:57:33Z | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | true | |
| workflow.import.source | pubmed | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Chemical%20Biology&rft.date=2015-06-18&rft.volume=22&rft.issue=6&rft.spage=712-23&rft.epage=712-23&rft.eissn=1879-1301&rft.issn=1879-1301&rft.au=THIERRY,%20Sylvain&BENLEULMI,%20Mohamed%20Salah&SINZELLE,%20Ludivine&THIERRY,%20Eloise&CALMELS,%20Christina&rft.genre=article |
