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Dual and Opposite Effects of hRAD51 Chemical Modulation on HIV-1 Integration.

dc.rights.licenseopenen_US
dc.contributor.authorTHIERRY, Sylvain
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBENLEULMI, Mohamed Salah
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorSINZELLE, Ludivine
dc.contributor.authorTHIERRY, Eloise
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorCALMELS, Christina
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCHAIGNEPAIN, Stephane
hal.structure.identifierGroupe d’Etudes des Substances Végétales à Activité Biologique [GESVAB ]
dc.contributor.authorWAFFO-TEGUO, Pierre
hal.structure.identifierGroupe d’Etudes des Substances Végétales à Activité Biologique [GESVAB ]
dc.contributor.authorMERILLON, Jean-Michel
dc.contributor.authorBUDKE, Brian
hal.structure.identifierINSERM U1035, Université de Bordeaux, UFR Sciences de la Vie et de la Santé, 33000 Bordeaux
dc.contributor.authorPASQUET, Jean-Max
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorLITVAK, Simon
dc.contributor.authorCIUFFI, Angela
dc.contributor.authorSUNG, Patrick
dc.contributor.authorCONNELL, Philip
dc.contributor.authorHAUBER, Ilona
dc.contributor.authorHAUBER, Joachim
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorANDREOLA, Marie-Line
dc.contributor.authorDELELIS, Olivier
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorPARISSI, Vincent
dc.date.accessioned2023-11-13T11:57:29Z
dc.date.available2023-11-13T11:57:29Z
dc.date.issued2015-06-18
dc.identifier.issn1879-1301en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/184728
dc.description.abstractEnThe cellular DNA repair hRAD51 protein has been shown to restrict HIV-1 integration both in vitro and in vivo. To investigate its regulatory functions, we performed a pharmacological analysis of the retroviral integration modulation by hRAD51. We found that, in vitro, chemical activation of hRAD51 stimulates its integration inhibitory properties, whereas inhibition of hRAD51 decreases the integration restriction, indicating that the modulation of HIV-1 integration depends on the hRAD51 recombinase activity. Cellular analyses demonstrated that cells exhibiting high hRAD51 levels prior to de novo infection are more resistant to integration. On the other hand, when hRAD51 was activated during integration, cells were more permissive. Altogether, these data establish the functional link between hRAD51 activity and HIV-1 integration. Our results highlight the multiple and opposite effects of the recombinase during integration and provide new insights into the cellular regulation of HIV-1 replication.
dc.language.isoENen_US
dc.subject.en4
dc.subject.en4'-Diisothiocyanostilbene-2
dc.subject.en2'-Disulfonic Acid
dc.subject.enAptamers
dc.subject.enNucleotide
dc.subject.enBenzamides
dc.subject.enDNA
dc.subject.enDNA Repair
dc.subject.enGene Expression
dc.subject.enHEK293 Cells
dc.subject.enHIV-1
dc.subject.enHumans
dc.subject.enMorpholines
dc.subject.enProtein Binding
dc.subject.enPyrroles
dc.subject.enRad51 Recombinase
dc.subject.enStilbenes
dc.subject.enSulfonamides
dc.subject.enVirus Internalization
dc.subject.enVirus Replication
dc.title.enDual and Opposite Effects of hRAD51 Chemical Modulation on HIV-1 Integration.
dc.title.alternativeChem Biolen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.chembiol.2015.04.020en_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologieen_US
dc.identifier.pubmed26051216en_US
bordeaux.journalJournal of Chemical Biologyen_US
bordeaux.page712-23en_US
bordeaux.volume22en_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue6en_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
hal.identifierhal-04282149
hal.version1
hal.date.transferred2023-11-13T11:57:33Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcepubmed
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Chemical%20Biology&rft.date=2015-06-18&rft.volume=22&rft.issue=6&rft.spage=712-23&rft.epage=712-23&rft.eissn=1879-1301&rft.issn=1879-1301&rft.au=THIERRY,%20Sylvain&BENLEULMI,%20Mohamed%20Salah&SINZELLE,%20Ludivine&THIERRY,%20Eloise&CALMELS,%20Christina&rft.genre=article


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