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Cloning, characterization and gene expression of a metallothionein isoform in the edible cockle Cerastoderma edule after cadmium or mercury exposure

dc.rights.licenseopenen_US
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorPAUL-PONT, Ika
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorGONZALEZ, Patrice
ORCID: 0000-0003-2628-2993
IDREF: 14341917X
dc.contributor.authorMONTERO, Natalia
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorDE MONTAUDOUIN, Xavier
IDREF: 075931664
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBAUDRIMONT, Magalie
IDREF: 105696722
dc.date.accessioned2023-10-16T12:56:20Z
dc.date.available2023-10-16T12:56:20Z
dc.date.issued2011-09-28
dc.identifier.issn0147-6513en_US
dc.identifier.urihttps://www.researchgate.net/publication/51685321_Cloning_characterization_and_gene_expression_of_a_metallothionein_isoform_in_the_edible_cockle_Cerastoderma_edule_after_cadmium_or_mercury_exposure
dc.identifier.urioai:researchgate.net:51685321
dc.identifier.urioai:crossref.org:10.1016/j.ecoenv.2011.08.025
dc.identifier.uri32941303
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/184440
dc.description.abstractEnMetallothionein (MT) genes encode crucial metal-binding proteins ubiquitously expressed in living organisms and which play important roles in homeostasis of essential metals and detoxification processes. Here, the molecular organization of the first metallothionein gene of the edible cockle Cerastoderma edule and its expression after cadmium (Cd) or mercury (Hg) exposures were determined. The resulting sequence (Cemt1) exhibits unusual features. The full length cDNA encodes a protein of 73 amino acids with nine classical Cys-X((1-3))-Cys motifs, but also one Cys-Cys not generally found in molluscan MT. Moreover, characterization of the molecular organization of the Cemt1 gene revealed two different alleles (A1 and A2) with length differences due to large deletion events in their intronic sequences involving direct Short Interspersed repeated Elements (SINE), while their exonic sequences were identical. To our knowledge, such large excision mechanisms have never before been reported in a bivalve gene sequence. After 10 days of Cd exposure at environmentally relevant doses, quantitative real-time PCR revealed a strong induction of Cemt1 in gills of C. edule. Surprisingly, neither induction of the Cemt1 gene nor of MT protein was shown after Hg exposure, despite the fact that this organism is able to bioaccumulate a high amount of this trace metal which is theoretically one of the most powerful inducers of MT biosynthesis.
dc.language.isoENen_US
dc.sourceresearchgate
dc.sourcecrossref
dc.sourceorcid
dc.title.enCloning, characterization and gene expression of a metallothionein isoform in the edible cockle Cerastoderma edule after cadmium or mercury exposure
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.ecoenv.2011.08.025en_US
dc.subject.halSciences de l'environnementen_US
bordeaux.journalEcotoxicology and Environmental Safetyen_US
bordeaux.page119-126en_US
bordeaux.volume75en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.teamECOBIOCen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.identifierhal-04244420
hal.version1
hal.date.transferred2023-10-16T12:56:22Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Ecotoxicology%20and%20Environmental%20Safety&rft.date=2011-09-28&rft.volume=75&rft.spage=119-126&rft.epage=119-126&rft.eissn=0147-6513&rft.issn=0147-6513&rft.au=PAUL-PONT,%20Ika&GONZALEZ,%20Patrice&MONTERO,%20Natalia&DE%20MONTAUDOUIN,%20Xavier&BAUDRIMONT,%20Magalie&rft.genre=article


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