PAUL-PONT, Ika; GONZALEZ, Patrice; MONTERO, Natalia; DE MONTAUDOUIN, Xavier; BAUDRIMONT, Magalie

doi:10.1016/j.ecoenv.2011.08.025

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

PAUL-PONT, Ika
Environnements et Paléoenvironnements OCéaniques [EPOC]

GONZALEZ, Patrice

Environnements et Paléoenvironnements OCéaniques [EPOC]

MONTERO, Natalia

Langue

Article de revue

Ce document a été publié dans

Ecotoxicology and Environmental Safety. 2011-09-28, vol. 75, p. 119-126

Résumé en anglais

Metallothionein (MT) genes encode crucial metal-binding proteins ubiquitously expressed in living organisms and which play important roles in homeostasis of essential metals and detoxification processes. Here, the molecular organization of the first metallothionein gene of the edible cockle Cerastoderma edule and its expression after cadmium (Cd) or mercury (Hg) exposures were determined. The resulting sequence (Cemt1) exhibits unusual features. The full length cDNA encodes a protein of 73 amino acids with nine classical Cys-X((1-3))-Cys motifs, but also one Cys-Cys not generally found in molluscan MT. Moreover, characterization of the molecular organization of the Cemt1 gene revealed two different alleles (A1 and A2) with length differences due to large deletion events in their intronic sequences involving direct Short Interspersed repeated Elements (SINE), while their exonic sequences were identical. To our knowledge, such large excision mechanisms have never before been reported in a bivalve gene sequence. After 10 days of Cd exposure at environmentally relevant doses, quantitative real-time PCR revealed a strong induction of Cemt1 in gills of C. edule. Surprisingly, neither induction of the Cemt1 gene nor of MT protein was shown after Hg exposure, despite the fact that this organism is able to bioaccumulate a high amount of this trace metal which is theoretically one of the most powerful inducers of MT biosynthesis.< Réduire

Métadonnées

Partager cette publication !

Licence d’utilisation du document

Cloning, characterization and gene expression of a metallothionein isoform in the edible cockle Cerastoderma edule after cadmium or mercury exposure

Langue

Ce document a été publié dans

Résumé en anglais

URI

DOI

Unités de recherche