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Intasome architecture and chromatin density modulate retroviral integration into nucleosome

hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorSALAH BENLEULMI, Mohamed
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorMATYSIAK, Julien
dc.contributor.authorRODRIGO HENRIQUEZ, Daniel
dc.contributor.authorVAILLANT, Cédric
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorLESBATS, Paul
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorCALMELS, Christina
dc.contributor.authorNAUGHTIN, Monica
dc.contributor.authorLEON, Oscar
dc.contributor.authorSKALKA, Anna
dc.contributor.authorRUFF, Marc
dc.contributor.authorLAVIGNE, Marc
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorANDREOLA, Marie-Line
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorPARISSI, Vincent
dc.date.accessioned2023-07-18T07:57:29Z
dc.date.available2023-07-18T07:57:29Z
dc.date.issued2015
dc.identifier.issn1742-4690
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/183416
dc.description.abstractEnBACKGROUND:Retroviral integration depends on the interaction between intasomes, host chromatin and cellular targeting cofactors as LEDGF/p75 or BET proteins. Previous studies indicated that the retroviral integrase, by itself, may play a role in the local integration site selection within nucleosomal target DNA. We focused our study on this local association by analyzing the intrinsic properties of various retroviral intasomes to functionally accommodate different chromatin structures in the lack of other cofactors.RESULTS:Using in vitro conditions allowing the efficient catalysis of full site integration without these cofactors, we show that distinct retroviral integrases are not equally affected by chromatin compactness. Indeed, while PFV and MLV integration reactions are favored into dense and stable nucleosomes, HIV-1 and ASV concerted integration reactions are preferred into poorly dense chromatin regions of our nucleosomal acceptor templates. Predicted nucleosome occupancy around integration sites identified in infected cells suggests the presence of a nucleosome at the MLV and HIV-1 integration sites surrounded by differently dense chromatin. Further analyses of the relationships between the in vitro integration site selectivity and the structure of the inserted DNA indicate that structural constraints within intasomes could account for their ability to accommodate nucleosomal DNA and could dictate their capability to bind nucleosomes functionally in these specific chromatin contexts.CONCLUSIONS:Thus, both intasome architecture and compactness of the chromatin surrounding the targeted nucleosome appear important determinants of the retroviral integration site selectivity. This supports a mechanism involving a global targeting of the intasomes toward suitable chromatin regions followed by a local integration site selection modulated by the intrinsic structural constraints of the intasomes governing the target DNA bending and dictating their sensitivity toward suitable specific nucleosomal structures and density
dc.language.isoen
dc.publisherBioMed Central
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.title.enIntasome architecture and chromatin density modulate retroviral integration into nucleosome
dc.typeArticle de revueen_US
dc.identifier.doi10.1186/s12977-015-0145-9
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]
bordeaux.page13
bordeaux.volume12
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
bordeaux.import.sourcehal
hal.identifierpasteur-01416843
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2015&rft.volume=12&rft.spage=13&rft.epage=13&rft.eissn=1742-4690&rft.issn=1742-4690&rft.au=SALAH%20BENLEULMI,%20Mohamed&MATYSIAK,%20Julien&RODRIGO%20HENRIQUEZ,%20Daniel&VAILLANT,%20C%C3%A9dric&LESBATS,%20Paul&rft.genre=article


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