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Structural basis for loading and inhibition of a bacterial T6 SS phospholipase effector by the VgrG spike
| dc.rights.license | open | en_US |
| dc.contributor.author | FLAUGNATTI, Nicolas | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | RAPISARDA, Chiara | |
| dc.contributor.author | REY, Martial | |
| dc.contributor.author | BEAUVOIS, Solène | |
| dc.contributor.author | NGUYEN, Viet Anh | |
| dc.contributor.author | CANAAN, Stéphane | |
| dc.contributor.author | DURAND, Eric | |
| dc.contributor.author | CHAMOT‐ROOKE, Julia | |
| dc.contributor.author | CASCALES, E. | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | FRONZES, Rémi | |
| dc.contributor.author | JOURNET, Laure | |
| dc.date.accessioned | 2023-06-02T10:31:25Z | |
| dc.date.available | 2023-06-02T10:31:25Z | |
| dc.date.issued | 2020-06-02 | |
| dc.identifier.issn | 0261-4189 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/182445 | |
| dc.description.abstractEn | The bacterial type VI secretion system (T6SS) is a macromolecular machine that injects effectors into prokaryotic and eukaryotic cells. The mode of action of the T6SS is similar to contractile phages: the contraction of a sheath structure pushes a tube topped by a spike into target cells. Effectors are loaded onto the spike or confined into the tube. In enteroaggregative Escherichia coli, the Tle1 phospholipase binds the C-terminal extension of the VgrG trimeric spike. Here, we purify the VgrG-Tle1 complex and show that a VgrG trimer binds three Tle1 monomers and inhibits their activity. Using covalent cross-linking coupled to high-resolution mass spectrometry, we provide information on the sites of contact and further identify the requirement for a Tle1 N-terminal secretion sequence in complex formation. Finally, we report the 2.6-Å-resolution cryo-electron microscopy tri-dimensional structure of the (VgrG) 3-(Tle1) 3 complex revealing how the effector binds its cargo, and how VgrG inhibits Tle1 phospholipase activity. The inhibition of Tle1 phospholipase activity once bound to VgrG suggests that Tle1 dissociation from VgrG is required upon delivery. | |
| dc.description.sponsorship | Contribution des Effecteurs du Système de Sécrétion de Type VI à la pathogénicité des Escherichia coli adhérents-invasifs | en_US |
| dc.description.sponsorship | Guerre bactérienne: Architecture et Fonction du Système de Sécrétion de Type VI - ANR-14-CE14-0006 | en_US |
| dc.description.sponsorship | Bloc-outil et Imagerie de Précision pour le Binage Intra-rang Précoce - ANR-17-ROSE-0001 | en_US |
| dc.description.sponsorship | Cibler le métabolisme des fibroblastes adventitiels activés pour traiter l'hypertension pulmonaire - ANR-20-CE14-0006 | en_US |
| dc.language.iso | EN | en_US |
| dc.subject.en | type VI secretion system | |
| dc.subject.en | Structural Biology | |
| dc.subject.en | Virology & Host Pathogen Interaction | |
| dc.subject.en | type VI secretion system Subject Categories Microbiology | |
| dc.subject.en | protein secretion | |
| dc.subject.en | cryo-electron microscopy | |
| dc.subject.en | bacterial toxin | |
| dc.subject.en | bacterial competition | |
| dc.title.en | Structural basis for loading and inhibition of a bacterial T6 SS phospholipase effector by the VgrG spike | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.15252/embj.2019104129 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio] | en_US |
| dc.description.sponsorshipEurope | Infrastructure for NMR, EM and X-ray crystallography for translational research | en_US |
| bordeaux.journal | EMBO Journal | en_US |
| bordeaux.page | e104129 | en_US |
| bordeaux.volume | 39 | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.issue | 11 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | hal | |
| hal.identifier | hal-02915537 | |
| hal.version | 1 | |
| hal.export | false | |
| workflow.import.source | hal | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=EMBO%20Journal&rft.date=2020-06-02&rft.volume=39&rft.issue=11&rft.spage=e104129&rft.epage=e104129&rft.eissn=0261-4189&rft.issn=0261-4189&rft.au=FLAUGNATTI,%20Nicolas&RAPISARDA,%20Chiara&REY,%20Martial&BEAUVOIS,%20Sol%C3%A8ne&NGUYEN,%20Viet%20Anh&rft.genre=article |
