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Adsorption of arabinogalactan-proteins from Acacia gums (senegal and seyal) and its molecular fractions onto latex particles

dc.rights.licenseopenen_US
dc.contributor.authorRENARD, Didier
dc.contributor.authorDAVANTES, A.
dc.contributor.authorD’ORLANDO, Angelina
dc.contributor.authorCAHIER, K.
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorMOLINARI, Michael
dc.contributor.authorNIGEN, M.
dc.contributor.authorCHALIER, P.
dc.contributor.authorSANCHEZ, C.
dc.date.accessioned2023-05-24T11:53:04Z
dc.date.available2023-05-24T11:53:04Z
dc.date.issued2022-04-09
dc.identifier.issn0268-005Xen_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/182307
dc.description.abstractEnAdsorption of Acacia gums (GA) (A. senegal and A. seyal) and A. senegal molecular fractions, made of arabinogalactan-proteins (AGPs) with different molecular weights, onto latex particles, with different sizes and surface charges, was studied using molecular (fluorescence and nanoIR spectroscopies, microscopies including Atomic Force Microscopy) and mesoscopic (Dynamic Light Scattering, zeta potential, Asymmetrical flow field-flow fractionation) methods. Adsorption of GA onto latex particles revealed a complex mechanism of adsorption where the highest adsorption was observed when A. senegal and latex particles were negatively charged. The invert was observed for A. seyal where the maximum of adsorption was observed when gum and latex were oppositely charged. The duality of positive and negative charges on GA macromolecules together with conformational changes certainly explain the complex mechanism of adsorption. A mechanism where either the protein moiety either the sugars blocks adsorb is to be preferred. This mechanism seems to produce differences of behaviour due to viscoelastic properties of adsorbed layers, and differences of hydration due to differences of polarity of AGPs. The consequence of electrostatics in the adsorption process is a water release from the adsorbed layers and important gain in entropy. A fair agreement was found between protein content and adsorbed layer thickness of GA, with the formation of partial coverage, monolayer and multilayer films depending on GA concentration. Structural changes induced by GA adsorption onto latex particles was probed using fluorescence and nanoIR spectroscopies without giving clear evidence of conformational changes induced after GA adsorption. The results of this study highlighted that GA surface adsorption process depend not only on the protein moiety and electrostatic interactions but also on other parameters related to AGP hydration status. The protein structural accessibility, the molecular weight distribution, the AGPs intrinsic viscoelastic properties allowing structural rearrangements on the surface and spreading in order to form a viscoelastic film onto latex particles should also play a pivotal role in the adsorption process.
dc.language.isoENen_US
dc.title.enAdsorption of arabinogalactan-proteins from Acacia gums (senegal and seyal) and its molecular fractions onto latex particles
dc.title.alternativeFood Hydrocolloidsen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.foodhyd.2021.107360en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalFood Hydrocolloidsen_US
bordeaux.volume125en_US
bordeaux.hal.laboratoriesCBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.exportfalse
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Food%20Hydrocolloids&rft.date=2022-04-09&rft.volume=125&rft.eissn=0268-005X&rft.issn=0268-005X&rft.au=RENARD,%20Didier&DAVANTES,%20A.&D%E2%80%99ORLANDO,%20Angelina&CAHIER,%20K.&MOLINARI,%20Michael&rft.genre=article


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