Fatty acid oxidation participates in resistance to nutrient-depleted environments in the insect stages of Trypanosoma cruzi
| dc.rights.license | open | en_US |
| dc.contributor.author | SOUZA, Rodolpho Ornitz Oliveira | |
| dc.contributor.author | DAMASCENO, Flávia Silva | |
| dc.contributor.author | MARSICCOBETRE, Sabrina | |
| hal.structure.identifier | Centre de résonance magnétique des systèmes biologiques [CRMSB] | |
| dc.contributor.author | BIRAN, Marc | |
| dc.contributor.author | MURATA, Gilson | |
| dc.contributor.author | CURI, Rui | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | BRINGAUD, Frédéric | |
| dc.contributor.author | SILBER, Ariel Mariano | |
| dc.date.accessioned | 2023-05-23T10:28:07Z | |
| dc.date.available | 2023-05-23T10:28:07Z | |
| dc.date.issued | 2021-04-05 | |
| dc.identifier.issn | 1553-7366 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/182258 | |
| dc.description.abstractEn | Trypanosoma cruzi , the parasite causing Chagas disease, is a digenetic flagellated protist that infects mammals (including humans) and reduviid insect vectors. Therefore, T . cruzi must colonize different niches in order to complete its life cycle in both hosts. This fact determines the need of adaptations to face challenging environmental cues. The primary environmental challenge, particularly in the insect stages, is poor nutrient availability. In this regard, it is well known that T . cruzi has a flexible metabolism able to rapidly switch from carbohydrates (mainly glucose) to amino acids (mostly proline) consumption. Also established has been the capability of T . cruzi to use glucose and amino acids to support the differentiation process occurring in the insect, from replicative non-infective epimastigotes to non-replicative infective metacyclic trypomastigotes. However, little is known about the possibilities of using externally available and internally stored fatty acids as resources to survive in nutrient-poor environments, and to sustain metacyclogenesis. In this study, we revisit the metabolic fate of fatty acid breakdown in T . cruzi . Herein, we show that during parasite proliferation, the glucose concentration in the medium can regulate the fatty acid metabolism. At the stationary phase, the parasites fully oxidize fatty acids. [U- 14 C]-palmitate can be taken up from the medium, leading to CO 2 production. Additionally, we show that electrons are fed directly to oxidative phosphorylation, and acetyl-CoA is supplied to the tricarboxylic acid (TCA) cycle, which can be used to feed anabolic pathways such as the de novo biosynthesis of fatty acids. Finally, we show as well that the inhibition of fatty acids mobilization into the mitochondrion diminishes the survival to severe starvation, and impairs metacyclogenesis. | |
| dc.description.sponsorship | Voies métaboliques glycosomales non glycolytiques: nouvelles fonctions pour le développement et la virulence des trypanosomes - ANR-15-CE15-0025 | en_US |
| dc.description.sponsorship | Interactions métaboliques entre les adipocytes et les trypanosomes, un nouveau paradigme pour les trypanosomoses | en_US |
| dc.description.sponsorship | Alliance française contre les maladies parasitaires | en_US |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.title.en | Fatty acid oxidation participates in resistance to nutrient-depleted environments in the insect stages of Trypanosoma cruzi | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1371/journal.ppat.1009495 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Parasitologie | en_US |
| bordeaux.journal | PLoS Pathogens | en_US |
| bordeaux.page | e1009495 | en_US |
| bordeaux.volume | 17 | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.issue | 4 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | hal | |
| hal.identifier | hal-03216712 | |
| hal.version | 1 | |
| hal.export | false | |
| workflow.import.source | hal | |
| dc.rights.cc | CC BY | en_US |
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