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Genetic drivers of chromosomal integron stability

dc.rights.licenseopenen_US
dc.contributor.authorRICHARD, Egill
dc.contributor.authorDARRACQ, Baptiste
dc.contributor.authorLITTNER, Eloi
dc.contributor.authorVIT, Claire
dc.contributor.authorWHITEWAY, Clémence
dc.contributor.authorBOS, Julia
dc.contributor.authorFOURNES, Florian
dc.contributor.authorGARRISS, Geneviève
dc.contributor.authorCONTE, Valentin
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorLAPAILLERIE, Delphine
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorPARISSI, Vincent
dc.contributor.authorROUSSET, François
dc.contributor.authorSKOVGAARD, Ole
dc.contributor.authorBIKARD, David
dc.contributor.authorROCHA, Eduardo
dc.contributor.authorMAZEL, Didier
dc.contributor.authorLOOT, Céline
dc.date.accessioned2023-05-17T08:39:48Z
dc.date.available2023-05-17T08:39:48Z
dc.date.created2022-08-07
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/182190
dc.description.abstractEnThe integron is a bacterial recombination system that allows acquisition, stockpiling and expression of promoterless genes embedded in cassettes. Some integrons, like the one found in the second chromosome of Vibrio cholerae, can be particularly massive and contain hundreds of non-expressed cassettes. It is unclear how such genetic structures can be stabilized in bacterial genomes. Here, we reveal that the orientation of integrons toward replication within bacterial chromosomes is essential to their stability. Indeed, we show that upon inversion of the V. cholerae chromosomal integron, its plasticity is dramatically increased. This correlates with a strong growth defect which we show is mostly due to the excision of a particular type of cassettes bearing their own promoter and encoding toxin-antitoxin systems. This so called "abortive excision" of toxin-antitoxin systems can prevent the inversion of chromosomal integrons and the associated extensive loss of cassettes. Our analysis of the available sedentary chromosomal integrons in genome database show a robust correlation between the size of the cassette array and the number of toxin-antitoxin cassettes. This study thus provides a striking example of the relationship between genome organization, genome stability, and an emerging property of toxin-antitoxin systems.
dc.description.sponsorshipEtude de l'impact des intégrons chromosiques sur l'évolution des bactériesen_US
dc.description.sponsorshipIntegrative Biology of Emerging Infectious Diseasesen_US
dc.language.isoENen_US
dc.rights.urihttp://hal.archives-ouvertes.fr/licences/copyright/
dc.subject.enbacterial evolution and plasticity
dc.subject.engenome organization
dc.subject.engenetic stability
dc.subject.ensitespecific recombination
dc.subject.enmobile elements
dc.subject.ensedentary chromosomal integron
dc.subject.entoxin-antitoxin cassettes
dc.title.enGenetic drivers of chromosomal integron stability
dc.typeDocument de travail - Pré-publicationen_US
dc.identifier.doi10.1101/2022.08.03.502626en_US
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaireen_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologieen_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.institutionCNRSen_US
bordeaux.import.sourcehal
hal.identifierpasteur-03824759
hal.version1
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=RICHARD,%20Egill&DARRACQ,%20Baptiste&LITTNER,%20Eloi&VIT,%20Claire&WHITEWAY,%20Cl%C3%A9mence&rft.genre=preprint


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