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Deciphering RNA G-quadruplex function during the early steps of HIV-1 infection

dc.rights.licenseopenen_US
dc.contributor.authorAMRANE, Samir
dc.contributor.authorJAUBERT, Chloé
dc.contributor.authorBEDRAT, Amina
dc.contributor.authorRUNDSTADLER, Tiffany
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorRECORDON-PINSON, Patricia
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorAKNIN, Cindy
dc.contributor.authorGUÉDIN, Aurore
dc.contributor.authorDE RACHE, Aurore
dc.contributor.authorBARTOLUCCI, Laura
hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierAcides Nucléiques : Régulations Naturelle et Artificielle [ARNA]
dc.contributor.authorDIENE, Ibra
dc.contributor.authorÉRIC LEMOINE, Fréd
dc.contributor.authorGASCUEL, Olivier
dc.contributor.authorPRATVIEL, G.
dc.contributor.authorMERGNY, Jean-Louis
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorMARIE-LINE, Andreola
dc.date.accessioned2023-05-16T14:19:47Z
dc.date.available2023-05-16T14:19:47Z
dc.date.issued2022
dc.identifier.issn0305-1048en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/182161
dc.description.abstractEnG-quadruplexes (G4s) are four-stranded nucleic acid structures formed by the stacking of G-tetrads. Here we investigated their formation and function during HIV-1 infection. Using bioinformatics and biophysics analyses we first searched for evolutionary conserved G4-forming sequences in HIV-1 genome. We identified 10 G4s with conservation rates higher than those of HIV-1 regulatory sequences such as RRE and TAR. We then used porphyrin-based G4binders to probe the formation of the G4s during infection of human cells by native HIV-1. The G4binders efficiently inhibited HIV-1 infectivity, which is attributed to the formation of G4 structures during HIV-1 replication. Using a qRT-PCR approach, we showed that the formation of viral G4s occurs during the first 2 h post-infection and their stabilization by the G4-binders prevents initiation of reverse transcription. We also used a G4-RNA pull-down approach, based on a G4-specific biotinylated probe, to allow the direct detection and identification of viral G4-RNA in infected cells. Most of the detected G4-RNAs contain crucial regulatory elements such as the PPT and cPPT sequences as well as the U3 region. Hence, these G4s would function in the early stages of infection when the viral RNA genome is being processed for the reverse transcription step.
dc.description.sponsorshipPaRis Artificial Intelligence Research InstitutE - ANR-19-P3IA-0001en_US
dc.language.isoENen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc/
dc.title.enDeciphering RNA G-quadruplex function during the early steps of HIV-1 infection
dc.typeArticle de revueen_US
dc.identifier.doi10.1093/nar/gkac1030en_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologieen_US
bordeaux.journalNucleic Acids Researchen_US
bordeaux.page12328-12343en_US
bordeaux.volume50en_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue21en_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-03874577
hal.version1
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nucleic%20Acids%20Research&rft.date=2022&rft.volume=50&rft.issue=21&rft.spage=12328-12343&rft.epage=12328-12343&rft.eissn=0305-1048&rft.issn=0305-1048&rft.au=AMRANE,%20Samir&JAUBERT,%20Chlo%C3%A9&BEDRAT,%20Amina&RUNDSTADLER,%20Tiffany&RECORDON-PINSON,%20Patricia&rft.genre=article


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