Single-molecule and super-resolution microscopies of complex organizations for biological applications
| hal.structure.identifier | lp2n-04,lp2n-12 | |
| dc.contributor.author | COGNET, Laurent | |
| dc.date.accessioned | 2023-05-12T10:59:37Z | |
| dc.date.available | 2023-05-12T10:59:37Z | |
| dc.date.conference | 2014-11-05 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/182009 | |
| dc.description.abstractEn | The optical microscopy of single molecules has recently been beneficial for many applications, in particular in biology as acknowledged by the Nobel Prize of Chemistry in 2014. It allows a sub-wavelength localization of isolated molecules and subtle probing of their spatio-temporal nano-environments on living cells. It also allows designing innovative strategies to obtain super-resolved optical images i.e. with resolution below the diffraction limit.In a first part, after introducing “standard” single molecule detection strategies currently used in biology, I will present novel developments in the field. In particular, for many single-molecule microscopy applications, more photostable nanoprobes than fluorescent ones are desirable. For this aim, we developed several years ago far-field photothermal methods based on absorption instead of luminescence. Such approaches do not suffer from the inherent photophysical limitations of luminescent objects and allows the ultra-sensitive detection of tiny absorbing individual nano-objects such as gold nanoparticles down to 5 nm in cells or carbon nanotubes. I will present our current efforts to reduce the size of the functional nano-objects and thus obtain single-molecule photothermal nanoprobes for biomolecules found in confined cellular environment (adhesion sites, synapses etc...)The second part of my presentation will be devoted to the presentation of super-resolution microscopy methods. It is indeed crucial to study a large ensemble of molecules on a single cell while keeping the sub-wavelength localization provided by single molecule microscopy. In order to study the dynamical properties of endogenous membrane proteins found at high densities on living cells we developed a new single molecule super-resolution technique, named uPAINT. Interestingly, uPAINT does not require the use of photo-activable dyes allowing easy multi-color super-resolution imaging and single molecule tracking. Different applications of uPAINT will be presented, in particularly the first demonstration of super-resolution imaging of functional receptors in interaction. This last result was obtained combining super-resolution microscopy and single molecule FRET. | |
| dc.language.iso | en | |
| dc.title.en | Single-molecule and super-resolution microscopies of complex organizations for biological applications | |
| dc.type | Communication dans un congrès avec actes | |
| dc.subject.hal | Physique [physics]/Physique [physics]/Biophysique [physics.bio-ph] | |
| bordeaux.hal.laboratories | Laboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298 | * |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.institution | CNRS | |
| bordeaux.country | FR | |
| bordeaux.title.proceeding | IMAGING A Workshop of the German-French PhD College “Membranes and Membrane Proteins” UFA 0407 | |
| bordeaux.conference.city | Strasbourg | |
| bordeaux.peerReviewed | non | |
| hal.identifier | hal-01223500 | |
| hal.version | 1 | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-01223500v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=COGNET,%20Laurent&rft.genre=proceeding |
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