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hal.structure.identifierMax Planck Institute for Biophysical Chemistry [MPI-BPC]
dc.contributor.authorHELL, Stefan
hal.structure.identifierMax Planck Institute for Biophysical Chemistry [MPI-BPC]
dc.contributor.authorSAHL, Steffen
hal.structure.identifierMax Planck Institute for Biophysical Chemistry [MPI-BPC]
dc.contributor.authorBATES, Mark
hal.structure.identifierHoward Hughes Medical Institute [HHMI]
dc.contributor.authorZHUANG, Xiaowei
hal.structure.identifierFriedrich-Schiller-Universität = Friedrich Schiller University Jena [Jena, Germany]
dc.contributor.authorHEINTZMANN, Rainer
hal.structure.identifierDepartment of Engineering Science
dc.contributor.authorBOOTH, Martin J
hal.structure.identifierInstitute for Molecular Biophysics
dc.contributor.authorBEWERSDORF, Joerg
hal.structure.identifierJanelia Farm Research Campus
dc.contributor.authorSHTENGEL, Gleb
hal.structure.identifierJanelia Farm Research Campus
dc.contributor.authorHESS, Harald
hal.structure.identifierInstitute of Physical and Theoretical Chemistry [Braunschweig]
hal.structure.identifierBraunschweig Integrated Centre of Systems Biology [Braunschweig] [BRICS]
hal.structure.identifierLaboratory for Emerging Nanotechnology [LENA]
dc.contributor.authorTINNEFELD, Philipp
hal.structure.identifierMax Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG]
dc.contributor.authorHONIGMANN, Alf
hal.structure.identifierDepartment of NanoBiophotonics [Göttingen]
dc.contributor.authorJAKOBS, Stefan
hal.structure.identifierDepartment of NanoBiophotonics [Göttingen]
dc.contributor.authorTESTA, Ilaria
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorCOGNET, Laurent
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorLOUNIS, Brahim
hal.structure.identifierFreie Universität Berlin
dc.contributor.authorEWERS, Helge
hal.structure.identifierNuffield Department of Clinical Medicine [Oxford]
dc.contributor.authorDAVIS, Simon J
hal.structure.identifierMRC Human Immunology Unit
dc.contributor.authorEGGELING, Christian
hal.structure.identifierDepartment of Chemistry [Cambridge, UK]
dc.contributor.authorKLENERMAN, David
hal.structure.identifierCenter for Nanoscale Microscopy and Molecular Physiology of the Brain, Göttingen, Germany
dc.contributor.authorWILLIG, Katrin
hal.structure.identifierNanoscopy, Nanophysics, IstitutoItaliano di Tecnologia, Via Morego, 30, 16163, Genoa, Italy
dc.contributor.authorVICIDOMINI, Giuseppe
hal.structure.identifierNanoscopy, Nanophysics, IstitutoItaliano di Tecnologia, Via Morego, 30, 16163, Genoa, Italy
dc.contributor.authorCASTELLO, Marco
hal.structure.identifierNanoscopy, Nanophysics, IstitutoItaliano di Tecnologia, Via Morego, 30, 16163, Genoa, Italy
dc.contributor.authorDIASPRO, Alberto
dc.contributor.authorCORDES, Thorben
dc.contributor.authorSAHL, Steffen J
dc.contributor.authorTINNEFELD, Philip
dc.contributor.authorKLENERMAN, David
dc.contributor.authorWILLIG, Katrin I
dc.date.accessioned2023-05-12T10:51:57Z
dc.date.available2023-05-12T10:51:57Z
dc.date.issued2015-10-14
dc.identifier.issn0022-3727
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181850
dc.description.abstractEnFar-field optical microscopy using focused light is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study of living cells and organisms. Unfortunately, the applicability of the optical microscope is limited, since the diffraction of light imposes limitations on the spatial resolution of the image. Consequently the details of, for example, cellular protein distributions, can be visualized only to a certain extent. Fortunately, recent years have witnessed the development of 'super-resolution' far-field optical microscopy (nanoscopy) techniques such as stimulated emission depletion (STED), ground state depletion (GSD), reversible saturated optical (fluorescence) transitions (RESOLFT), photoactivation localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM) or saturated structured illumination microscopy (SSIM), all in one way or another addressing the problem of the limited spatial resolution of far-field optical microscopy. While SIM achieves a two-fold improvement in spatial resolution compared to conventional optical microscopy, STED, RESOLFT, PALM/STORM, or SSIM have all gone beyond, pushing the limits of optical image resolution to the nanometer scale. Consequently, all super-resolution techniques open new avenues of biomedical research. Because the field is so young, the potential capabilities of different super-resolution microscopy approaches have yet to be fully explored, and uncertainties remain when considering the best choice of methodology. Thus, even for experts, the road to the future is sometimes shrouded in mist. The super-resolution optical microscopy roadmap of Journal of Physics D: Applied Physics addresses this need for clarity. It provides guidance to the outstanding questions through a collection of short review articles from experts in the field, giving a thorough discussion on the concepts underlying super-resolution optical microscopy, the potential of different approaches, the importance of label optimization (such as reversible photoswitchable proteins) and applications in which these methods will have a significant impact.
dc.language.isoen
dc.publisherIOP Publishing
dc.titleSuper-resolution microscopy in Neurosciences: zoom in on synapses – Laurent
dc.title.enThe 2015 super-resolution microscopy roadmap
dc.typeArticle de revue
dc.identifier.doi10.1088/0022-3727/48/44/443001
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
dc.identifier.arxiv1711.04999
bordeaux.journalJournal of Physics D: Applied Physics
bordeaux.page443001
bordeaux.volume48
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.issue44
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-01390051
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01390051v1
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