Afficher la notice abrégée

Three-dimensional nanometre localization of nanoparticles to enhance super-resolution microscopy

hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
hal.structure.identifierInstitut Langevin - Ondes et Images (UMR7587) [IL]
hal.structure.identifierInstitut des Sciences Moléculaires d'Orsay [ISMO]
dc.contributor.authorBON, Pierre
hal.structure.identifierInstitut des Sciences Moléculaires d'Orsay [ISMO]
dc.contributor.authorBOURG, Nicolas
hal.structure.identifierCentre de Photonique Biomédicale [CPBM]
dc.contributor.authorLÉCART, Sandrine
hal.structure.identifierMOSAIC [MOSAIC]
dc.contributor.authorMONNERET, Serge
hal.structure.identifierInstitut Langevin - Ondes et Images (UMR7587) [IL]
dc.contributor.authorFORT, Emmanuel
hal.structure.identifierMOSAIC [MOSAIC]
dc.contributor.authorWENGER, Jérôme
hal.structure.identifierInstitut des Sciences Moléculaires d'Orsay [ISMO]
dc.contributor.authorLÉVÊQUE-FORT, Sandrine
dc.date.accessioned2023-05-12T10:42:48Z
dc.date.available2023-05-12T10:42:48Z
dc.date.issued2015-07-27
dc.identifier.issn2041-1723
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181663
dc.description.abstractEnMeeting the nanometre resolution promised by super-resolution microscopy techniques (pointillist: PALM, STORM, scanning: STED) requires stabilizing the sample drifts in real time during the whole acquisition process. Metal nanoparticles are excellent probes to track the lateral drifts as they provide crisp and photostable information. However, achieving nano-metre axial super-localization is still a major challenge, as diffraction imposes large depths-of-fields. Here we demonstrate fast full three-dimensional nanometre super-localization of gold nanoparticles through simultaneous intensity and phase imaging with a wavefront-sensing camera based on quadriwave lateral shearing interferometry. We show how to combine the intensity and phase information to provide the key to the third axial dimension. Presently, we demonstrate even in the occurrence of large three-dimensional fluctuations of several microns, unprecedented sub-nanometre localization accuracies down to 0.7 nm in lateral and 2.7 nm in axial directions at 50 frames per second. We demonstrate that nanoscale stabilization greatly enhances the image quality and resolution in direct stochastic optical reconstruction microscopy imaging.
dc.description.sponsorshipInstitut Langevin : Ondes et Images, du Fondamental à l'Innovation - ANR-10-LABX-0024
dc.description.sponsorshipParis Sciences et Lettres - ANR-10-IDEX-0001
dc.description.sponsorshipMicroscopie de fluorescence pour l'imagerie membranaire - ANR-11-EMMA-0047
dc.language.isoen
dc.publisherNature Publishing Group
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enNanoparticles
dc.subject.enSuper-resolution microscopy
dc.title.enThree-dimensional nanometre localization of nanoparticles to enhance super-resolution microscopy
dc.typeArticle de revue
dc.identifier.doi10.1038/ncomms8764
dc.subject.halPhysique [physics]/Physique [physics]/Optique [physics.optics]
dc.subject.halSciences de l'ingénieur [physics]/Optique / photonique
dc.subject.halSciences de l'ingénieur [physics]/Micro et nanotechnologies/Microélectronique
bordeaux.journalNature Communications
bordeaux.page7764
bordeaux.volume6
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-01182402
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01182402v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nature%20Communications&rft.date=2015-07-27&rft.volume=6&rft.spage=7764&rft.epage=7764&rft.eissn=2041-1723&rft.issn=2041-1723&rft.au=BON,%20Pierre&BOURG,%20Nicolas&L%C3%89CART,%20Sandrine&MONNERET,%20Serge&FORT,%20Emmanuel&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée