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Can phase masks extend depth-of-field in localization microscopy?

hal.structure.identifierLaboratoire Charles Fabry / Imagerie et Information
dc.contributor.authorLÉVÊQUE, Olivier
hal.structure.identifierLaboratoire Charles Fabry / Imagerie et Information
dc.contributor.authorKULCSÁR, Caroline
hal.structure.identifierLaboratoire Charles Fabry / Imagerie et Information
dc.contributor.authorSAUER, Hervé
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorLEE, Antony
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorBON, Pierre
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorCOGNET, Laurent
hal.structure.identifierLaboratoire Charles Fabry / Imagerie et Information
dc.contributor.authorGOUDAIL, François
dc.date.accessioned2023-05-12T10:37:56Z
dc.date.available2023-05-12T10:37:56Z
dc.date.issued2020-10-13
dc.date.conference2020-04-06
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181544
dc.description.abstractEnIn localization microscopy, the position of isolated fluorescent emitters are estimated with a resolution better than the diffraction limit. In order to image thick samples, which are common in biological applications, there is considerable interest in extending the depth-of-field of such microscopes in order to make their accuracy as invariant as possible to defocus. For that purpose, we propose to optimize annular binary phase masks placed in the pupil of the microscope in order to generate a point spread function for which the localization accuracy is almost invariant along the optical axis. The optimization criterion is defined as the localization accuracy in the plane expressed in terms of the Cramér-Rao bound. We show that the optimal masks significantly increase the depth-of-field of single-molecule imaging techniques relatively to an usual microscope objective.
dc.language.isoen
dc.publisherSPIE
dc.title.enCan phase masks extend depth-of-field in localization microscopy?
dc.typeCommunication dans un congrès avec actes
dc.identifier.doi10.1117/12.2558426
dc.subject.halPhysique [physics]/Physique [physics]/Optique [physics.optics]
dc.subject.halSciences de l'ingénieur [physics]/Traitement du signal et de l'image
bordeaux.page50-58
bordeaux.volume11351
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.countryFR
bordeaux.title.proceedingUnconventional Optical Imaging II
bordeaux.conference.cityOnline Only
bordeaux.peerReviewedoui
hal.identifierhal-03025685
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03025685v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2020-10-13&rft.volume=11351&rft.spage=50-58&rft.epage=50-58&rft.au=L%C3%89V%C3%8AQUE,%20Olivier&KULCS%C3%81R,%20Caroline&SAUER,%20Herv%C3%A9&LEE,%20Antony&BON,%20Pierre&rft.genre=proceeding


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