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Detection of all adult Tau isoforms in a 3D culture model of iPSC-derived neurons

hal.structure.identifierGénomique et Médecine Personnalisée du Cancer et des Maladies Neuropsychiatriques [GPMCND]
dc.contributor.authorMIGUEL, Laetitia
hal.structure.identifierGénomique et Médecine Personnalisée du Cancer et des Maladies Neuropsychiatriques [GPMCND]
dc.contributor.authorROVELET-LECRUX, Anne
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorFEYEUX, Maxime
hal.structure.identifierGénomique et Médecine Personnalisée du Cancer et des Maladies Neuropsychiatriques [GPMCND]
dc.contributor.authorFREBOURG, Thierry
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorNASSOY, Pierre
hal.structure.identifierGénomique et Médecine Personnalisée du Cancer et des Maladies Neuropsychiatriques [GPMCND]
dc.contributor.authorCAMPION, Dominique
hal.structure.identifierGénomique et Médecine Personnalisée du Cancer et des Maladies Neuropsychiatriques [GPMCND]
dc.contributor.authorLECOURTOIS, Magalie
dc.date.accessioned2023-05-12T10:37:30Z
dc.date.available2023-05-12T10:37:30Z
dc.date.issued2019-10
dc.identifier.issn1876-7753
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181538
dc.description.abstractEnTauopathies are a class of neurodegenerative diseases characterized by the presence of pathological intracellular deposits of Tau proteins. Six isoforms of Tau are expressed in the adult human brain, resulting from alternative splicing of the MAPT gene. Tau splicing is developmentally regulated such that only the smallest Tau isoform is expressed in fetal brain, contrary to the adult brain showing the expression of all 6 isoforms. Induced Pluripotent Stem Cell (iPSC) technology has opened up new perspectives in human disease modeling, including tauopathies. However, a major challenge to in vitro recapitulation of Tau pathology in iPSC-derived neurons is their relative immaturity. In this study, we examined the switch in Tau splicing from fetal-only to all adult Tau isoforms during the differentiation of iPSC-derived neurons in a new 3D culture system. First, we showed that iPSC-induced neurons inside Matrigel-coated alginate capsules were able to differentiate into cortical neurons. Then, using a new assay that allowed both the qualitative and the quantitative analysis of all adult MAPT mRNA isoforms individually, we demonstrated that BrainPhys-maintained neurons expressed the 6 adult MAPT mRNA transcripts from 25 weeks of maturation, making this model highly suitable for modeling Tau pathology and therapeutic purposes.
dc.language.isoen
dc.publisherElsevier
dc.title.enDetection of all adult Tau isoforms in a 3D culture model of iPSC-derived neurons
dc.typeArticle de revue
dc.identifier.doi10.1016/j.scr.2019.101541
dc.subject.halSciences du Vivant [q-bio]/Neurosciences [q-bio.NC]/Neurobiologie
bordeaux.journalStem Cell Research
bordeaux.page101541
bordeaux.volume40
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-03038792
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03038792v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Stem%20Cell%20Research&rft.date=2019-10&rft.volume=40&rft.spage=101541&rft.epage=101541&rft.eissn=1876-7753&rft.issn=1876-7753&rft.au=MIGUEL,%20Laetitia&ROVELET-LECRUX,%20Anne&FEYEUX,%20Maxime&FREBOURG,%20Thierry&NASSOY,%20Pierre&rft.genre=article


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