Show simple item record

The Incubascope : a simple, compact and large field of view microscope for long-term imaging inside an incubator

hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
hal.structure.identifierInstitut d'Optique Graduate School [IOGS]
hal.structure.identifierCentre National de la Recherche Scientifique [CNRS]
hal.structure.identifierUniversité de Bordeaux [UB]
dc.contributor.authorBADON, Amaury
hal.structure.identifierUniversité de Bordeaux [UB]
dc.contributor.authorANDRIQUE, Laetitia
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
hal.structure.identifierInstitut d'Optique Graduate School [IOGS]
hal.structure.identifierCentre National de la Recherche Scientifique [CNRS]
hal.structure.identifierUniversité de Bordeaux [UB]
dc.contributor.authorMOMBEREAU, Amaël
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
hal.structure.identifierInstitut d'Optique Graduate School [IOGS]
hal.structure.identifierCentre National de la Recherche Scientifique [CNRS]
hal.structure.identifierUniversité de Bordeaux [UB]
dc.contributor.authorRIVET, Louis
hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorBOYREAU, Adeline
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
hal.structure.identifierInstitut d'Optique Graduate School [IOGS]
hal.structure.identifierCentre National de la Recherche Scientifique [CNRS]
hal.structure.identifierUniversité de Bordeaux [UB]
dc.contributor.authorNASSOY, Pierre
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
hal.structure.identifierInstitut d'Optique Graduate School [IOGS]
hal.structure.identifierCentre National de la Recherche Scientifique [CNRS]
hal.structure.identifierUniversité de Bordeaux [UB]
dc.contributor.authorRECHER, Gaëlle
dc.date.accessioned2023-05-12T10:35:01Z
dc.date.available2023-05-12T10:35:01Z
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181483
dc.description.abstractEnOptical imaging has rapidly evolved in the last decades. Sophisticated microscopes allowing optical sectioning for 3D imaging or sub-diffraction resolution are available. Due to price and maintenance issues, these microscopes are often shared between users in facilities. Consequently, long term access is often prohibited and does not allow to monitor slowly evolving biological systems or to validate new models like organoids. Preliminary coarse long-term data that do not require acquisition of terabytes of high-resolution images are important as a first step. By contrast with expansive all-in-one commercialized stations, standard microscopes equipped with incubator stages offer a more cost-effective solution despite imperfect long run atmosphere and temperature control. Here, we present the Incubascope, a custom-made compact microscope that fits into a table-top incubator. It is cheap and simple to implement, user-friendly and yet provides high imaging performances. The system has a field of view of 5.5 × 8 mm 2 , a 3 µm resolution, a 10 frames/second acquisition rate, and is controlled with a Python-based graphical interface. We exemplify the capabilities of the Incubascope on biological applications such as the hatching of Artemia salina eggs, the growth of the slime mold Physarum polycephalum and of encapsulated spheroids of mammalian cells.
dc.description.sponsorshipMécanotransduction des cellules souches adipeuses et applications pour l'ingénierie tissulaire - ANR-17-CE13-0012
dc.description.sponsorshipElasticité et Dissipation des tissus en croissance - ANR-17-CE30-0007
dc.description.sponsorshipValidation translationnelle des greffes d'organoïdes neuraux pour le traitement des maladies de Parkinson et de Huntington - ANR-17-CE18-0026
dc.language.isoen
dc.title.enThe Incubascope : a simple, compact and large field of view microscope for long-term imaging inside an incubator
dc.typeDocument de travail - Pré-publication
dc.identifier.doi10.1101/2021.09.21.461183
dc.subject.halPhysique [physics]/Physique [physics]/Optique [physics.optics]
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
hal.identifierhal-03396414
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03396414v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=BADON,%20Amaury&ANDRIQUE,%20Laetitia&MOMBEREAU,%20Ama%C3%ABl&RIVET,%20Louis&BOYREAU,%20Adeline&rft.genre=preprint


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record