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A novel 3D culture model recapitulates primary FL B cell features and promotes their survival

hal.structure.identifierMicroenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
hal.structure.identifierEtablissement français du sang [Rennes] [EFS Bretagne]
dc.contributor.authorLAMAISON, Claire
hal.structure.identifierUniversity of Toronto
dc.contributor.authorLATOUR, Simon
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorHÉLAINE, Nelson
hal.structure.identifierActions for OnCogenesis understanding and Target Identification in ONcology [ACTION]
dc.contributor.authorLE MORVAN, Valerie
hal.structure.identifierMicroenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
hal.structure.identifierEtablissement français du sang [Rennes] [EFS Bretagne]
dc.contributor.authorSAINT-VANNE, Julien
hal.structure.identifierActions for OnCogenesis understanding and Target Identification in ONcology [ACTION]
dc.contributor.authorMAHOUCHE, Isabelle
hal.structure.identifierMicroenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
dc.contributor.authorMONVOISIN, Celine
hal.structure.identifierActions for OnCogenesis understanding and Target Identification in ONcology [ACTION]
dc.contributor.authorDUSSERT, Christelle
hal.structure.identifierUniversité de Bordeaux [UB]
dc.contributor.authorANDRIQUE, Laëtitia
hal.structure.identifierMicroenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
hal.structure.identifierEtablissement français du sang [Rennes] [EFS Bretagne]
dc.contributor.authorDELEURME, Laurent
hal.structure.identifierMicroenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
dc.contributor.authorDESSAUGE, Elise
hal.structure.identifierUniversité de Rennes [UR]
dc.contributor.authorPANGAULT, Celine
hal.structure.identifierInstitut Curie [Paris]
dc.contributor.authorBAULANDE, Sylvain
hal.structure.identifierInstitut Curie [Paris]
hal.structure.identifierPlateforme de sequencage ICGEX
dc.contributor.authorLEGOIX, Patricia
hal.structure.identifierUniversité de Rennes [UR]
dc.contributor.authorSEFFALS, Marine
hal.structure.identifierActions for OnCogenesis understanding and Target Identification in ONcology [ACTION]
dc.contributor.authorBROCA-BRISSON, Lea
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorALESSANDRI, Kévin
hal.structure.identifierBordeaux Research In Translational Oncology [Bordeaux] [BaRITOn]
dc.contributor.authorPROCHAZKOVA-CARLOTTI, Martina
hal.structure.identifierInstitut Bergonié [Bordeaux]
dc.contributor.authorSOUBEYRAN, Pierre
hal.structure.identifierCHU Bordeaux
dc.contributor.authorMERLIO, Jean-Philippe
hal.structure.identifierMicroenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
hal.structure.identifierEtablissement français du sang [Rennes] [EFS Bretagne]
dc.contributor.authorMOURCIN, Frédéric
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorNASSOY, Pierre
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorRECHER, Gaëlle
hal.structure.identifierMicroenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
hal.structure.identifierEtablissement français du sang [Rennes] [EFS Bretagne]
dc.contributor.authorTARTE, Karin
hal.structure.identifierBordeaux Research In Translational Oncology [Bordeaux] [BaRITOn]
dc.contributor.authorBRESSON-BEPOLDIN, Laurence
dc.date.accessioned2023-05-12T10:34:37Z
dc.date.available2023-05-12T10:34:37Z
dc.date.issued2021-09-23
dc.identifier.issn2473-9529
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181478
dc.description.abstractEnNon-Hodgkin B-cell lymphomas (B-NHL) mainly develop within lymph nodes (LN) as densely packed aggregates of tumor cells and their surrounding microenvironment, creating a tumor niche specific to each lymphoma subtypes. In vitro preclinical models mimicking biomechanical forces, cellular microenvironment, and 3D organization of B-cell lymphomas remain scarce, while all these parameters constitute key determinants of lymphomagenesis and drug resistance. Using a microfluidic method based on cell encapsulation inside permeable, elastic, and hollow alginate microspheres, we developed a new tunable 3D-model incorporating lymphoma B cells, extracellular matrix (ECM), and/or tonsil stromal cells (TSC). We revealed that under 3D confinement lymphoma B cells were able to form cohesive spheroids resulting from overexpression of ECM components. Moreover, lymphoma B cells and TSC dynamically formed self-organized 3D spheroids favoring spheroid growth. 3D culture induced resistance to classical chemotherapeutic agent doxorubicin, but not to BCL2 inhibitor ABT-199, identifying this approach as a relevant in vitro model to assess the activity of therapeutic agents in B-NHL. RNAseq analysis highlighted the synergy of 3D, ECM, and TSC in upregulating similar pathways in malignant B cells in vitro than those overexpressed in primary lymphoma cells in situ. Finally, our 3D model including ECM and TSC allowed long-term in vitro survival of primary follicular lymphoma B cells. In conclusion, we propose a new high throughput 3D model mimicking lymphoma tumor niche and making it possible to study the dynamic relationship between lymphoma B cells and their microenvironment and to screen new anti-cancer drugs.
dc.description.sponsorshipDéveloppement d'une Plateforme Nationale pour la médecine régénératrice
dc.description.sponsorshipDéveloppment d'une infrastructure française distribuée coordonnée
dc.description.sponsorshipEquipement de biologie intégrative du cancer pour une médecine personnalisée
dc.language.isoen
dc.publisherThe American Society of Hematology
dc.rights.urihttp://creativecommons.org/licenses/by-nc/
dc.subject.enalginates
dc.subject.enb-lymphocytes
dc.subject.enlymphoma
dc.subject.enb-cell lymphomas
dc.subject.enneoplasms
dc.subject.enantineoplastic agents
dc.subject.enbcl-2 protein
dc.subject.encancer
dc.subject.endoxorubicin
dc.subject.enfollicular lymphoma
dc.title.enA novel 3D culture model recapitulates primary FL B cell features and promotes their survival
dc.typeArticle de revue
dc.identifier.doi10.1182/bloodadvances.2020003949
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Cancer
bordeaux.journalBlood Advances
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-03367891
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03367891v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Blood%20Advances&rft.date=2021-09-23&rft.eissn=2473-9529&rft.issn=2473-9529&rft.au=LAMAISON,%20Claire&LATOUR,%20Simon&H%C3%89LAINE,%20Nelson&LE%20MORVAN,%20Valerie&SAINT-VANNE,%20Julien&rft.genre=article


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