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A novel 3D culture model recapitulates primary FL B cell features and promotes their survival
hal.structure.identifier | Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC] | |
hal.structure.identifier | Etablissement français du sang [Rennes] [EFS Bretagne] | |
dc.contributor.author | LAMAISON, Claire | |
hal.structure.identifier | University of Toronto | |
dc.contributor.author | LATOUR, Simon | |
hal.structure.identifier | Laboratoire Photonique, Numérique et Nanosciences [LP2N] | |
dc.contributor.author | HÉLAINE, Nelson | |
hal.structure.identifier | Actions for OnCogenesis understanding and Target Identification in ONcology [ACTION] | |
dc.contributor.author | LE MORVAN, Valerie | |
hal.structure.identifier | Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC] | |
hal.structure.identifier | Etablissement français du sang [Rennes] [EFS Bretagne] | |
dc.contributor.author | SAINT-VANNE, Julien | |
hal.structure.identifier | Actions for OnCogenesis understanding and Target Identification in ONcology [ACTION] | |
dc.contributor.author | MAHOUCHE, Isabelle | |
hal.structure.identifier | Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC] | |
dc.contributor.author | MONVOISIN, Celine | |
hal.structure.identifier | Actions for OnCogenesis understanding and Target Identification in ONcology [ACTION] | |
dc.contributor.author | DUSSERT, Christelle | |
hal.structure.identifier | Université de Bordeaux [UB] | |
dc.contributor.author | ANDRIQUE, Laëtitia | |
hal.structure.identifier | Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC] | |
hal.structure.identifier | Etablissement français du sang [Rennes] [EFS Bretagne] | |
dc.contributor.author | DELEURME, Laurent | |
hal.structure.identifier | Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC] | |
dc.contributor.author | DESSAUGE, Elise | |
hal.structure.identifier | Université de Rennes [UR] | |
dc.contributor.author | PANGAULT, Celine | |
hal.structure.identifier | Institut Curie [Paris] | |
dc.contributor.author | BAULANDE, Sylvain | |
hal.structure.identifier | Institut Curie [Paris] | |
hal.structure.identifier | Plateforme de sequencage ICGEX | |
dc.contributor.author | LEGOIX, Patricia | |
hal.structure.identifier | Université de Rennes [UR] | |
dc.contributor.author | SEFFALS, Marine | |
hal.structure.identifier | Actions for OnCogenesis understanding and Target Identification in ONcology [ACTION] | |
dc.contributor.author | BROCA-BRISSON, Lea | |
hal.structure.identifier | Laboratoire Photonique, Numérique et Nanosciences [LP2N] | |
dc.contributor.author | ALESSANDRI, Kévin | |
hal.structure.identifier | Bordeaux Research In Translational Oncology [Bordeaux] [BaRITOn] | |
dc.contributor.author | PROCHAZKOVA-CARLOTTI, Martina | |
hal.structure.identifier | Institut Bergonié [Bordeaux] | |
dc.contributor.author | SOUBEYRAN, Pierre | |
hal.structure.identifier | CHU Bordeaux | |
dc.contributor.author | MERLIO, Jean-Philippe | |
hal.structure.identifier | Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC] | |
hal.structure.identifier | Etablissement français du sang [Rennes] [EFS Bretagne] | |
dc.contributor.author | MOURCIN, Frédéric | |
hal.structure.identifier | Laboratoire Photonique, Numérique et Nanosciences [LP2N] | |
dc.contributor.author | NASSOY, Pierre | |
hal.structure.identifier | Laboratoire Photonique, Numérique et Nanosciences [LP2N] | |
dc.contributor.author | RECHER, Gaëlle | |
hal.structure.identifier | Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC] | |
hal.structure.identifier | Etablissement français du sang [Rennes] [EFS Bretagne] | |
dc.contributor.author | TARTE, Karin | |
hal.structure.identifier | Bordeaux Research In Translational Oncology [Bordeaux] [BaRITOn] | |
dc.contributor.author | BRESSON-BEPOLDIN, Laurence | |
dc.date.accessioned | 2023-05-12T10:34:37Z | |
dc.date.available | 2023-05-12T10:34:37Z | |
dc.date.issued | 2021-09-23 | |
dc.identifier.issn | 2473-9529 | |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/181478 | |
dc.description.abstractEn | Non-Hodgkin B-cell lymphomas (B-NHL) mainly develop within lymph nodes (LN) as densely packed aggregates of tumor cells and their surrounding microenvironment, creating a tumor niche specific to each lymphoma subtypes. In vitro preclinical models mimicking biomechanical forces, cellular microenvironment, and 3D organization of B-cell lymphomas remain scarce, while all these parameters constitute key determinants of lymphomagenesis and drug resistance. Using a microfluidic method based on cell encapsulation inside permeable, elastic, and hollow alginate microspheres, we developed a new tunable 3D-model incorporating lymphoma B cells, extracellular matrix (ECM), and/or tonsil stromal cells (TSC). We revealed that under 3D confinement lymphoma B cells were able to form cohesive spheroids resulting from overexpression of ECM components. Moreover, lymphoma B cells and TSC dynamically formed self-organized 3D spheroids favoring spheroid growth. 3D culture induced resistance to classical chemotherapeutic agent doxorubicin, but not to BCL2 inhibitor ABT-199, identifying this approach as a relevant in vitro model to assess the activity of therapeutic agents in B-NHL. RNAseq analysis highlighted the synergy of 3D, ECM, and TSC in upregulating similar pathways in malignant B cells in vitro than those overexpressed in primary lymphoma cells in situ. Finally, our 3D model including ECM and TSC allowed long-term in vitro survival of primary follicular lymphoma B cells. In conclusion, we propose a new high throughput 3D model mimicking lymphoma tumor niche and making it possible to study the dynamic relationship between lymphoma B cells and their microenvironment and to screen new anti-cancer drugs. | |
dc.description.sponsorship | Développement d'une Plateforme Nationale pour la médecine régénératrice | |
dc.description.sponsorship | Développment d'une infrastructure française distribuée coordonnée - ANR-10-INBS-0004 | |
dc.description.sponsorship | Equipement de biologie intégrative du cancer pour une médecine personnalisée | |
dc.language.iso | en | |
dc.publisher | The American Society of Hematology | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/ | |
dc.subject.en | alginates | |
dc.subject.en | b-lymphocytes | |
dc.subject.en | lymphoma | |
dc.subject.en | b-cell lymphomas | |
dc.subject.en | neoplasms | |
dc.subject.en | antineoplastic agents | |
dc.subject.en | bcl-2 protein | |
dc.subject.en | cancer | |
dc.subject.en | doxorubicin | |
dc.subject.en | follicular lymphoma | |
dc.title.en | A novel 3D culture model recapitulates primary FL B cell features and promotes their survival | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1182/bloodadvances.2020003949 | |
dc.subject.hal | Sciences du Vivant [q-bio] | |
dc.subject.hal | Sciences du Vivant [q-bio]/Cancer | |
bordeaux.journal | Blood Advances | |
bordeaux.hal.laboratories | Laboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298 | * |
bordeaux.institution | Université de Bordeaux | |
bordeaux.institution | CNRS | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-03367891 | |
hal.version | 1 | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-03367891v1 | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Blood%20Advances&rft.date=2021-09-23&rft.eissn=2473-9529&rft.issn=2473-9529&rft.au=LAMAISON,%20Claire&LATOUR,%20Simon&H%C3%89LAINE,%20Nelson&LE%20MORVAN,%20Valerie&SAINT-VANNE,%20Julien&rft.genre=article |
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