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Fluorescent sp3 Defect-Tailored Carbon Nanotubes Enable NIR-II Single Particle Imaging in Live Brain Slices at Ultra-Low Excitation Doses

hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorMANDAL, Amit Kumar
hal.structure.identifierChemistry and Biochemistry Department (University of Maryland)
dc.contributor.authorWU, Xiaojian
hal.structure.identifierInterdisciplinary Institute for Neuroscience / Institut interdisciplinaire de neurosciences [Bordeaux] [IINS]
dc.contributor.authorFERREIRA, Joana
hal.structure.identifierChemistry and Biochemistry Department (University of Maryland)
dc.contributor.authorKIM, Mijin
hal.structure.identifierChemistry and Biochemistry Department (University of Maryland)
dc.contributor.authorPOWELL, Lyndsey
hal.structure.identifierChemistry and Biochemistry Department (University of Maryland)
dc.contributor.authorKWON, Hyejin
hal.structure.identifierInterdisciplinary Institute for Neuroscience / Institut interdisciplinaire de neurosciences [Bordeaux] [IINS]
dc.contributor.authorGROC, Laurent
hal.structure.identifierChemistry and Biochemistry Department (University of Maryland)
dc.contributor.authorWANG, Yuhuang
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorCOGNET, Laurent
dc.date.accessioned2023-05-12T10:34:25Z
dc.date.available2023-05-12T10:34:25Z
dc.date.issued2020-12
dc.identifier.issn2045-2322
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181477
dc.description.abstractEnAbstract Cellular and tissue imaging in the second near-infrared window (NIR-II, ~1000–1350 nm) is advantageous for in vivo studies because of low light extinction by biological constituents at these wavelengths. However, deep tissue imaging at the single molecule sensitivity has not been achieved in the NIR-II window due to lack of suitable bio-probes. Single-walled carbon nanotubes have emerged as promising near-infrared luminescent molecular bio-probes; yet, their inefficient photoluminescence (quantum yield ~1%) drives requirements for sizeable excitation doses (~1–10 kW/cm 2 ) that are significantly blue-shifted from the NIR-II region (<850 nm) and may thus ultimately compromise live tissue. Here, we show that single nanotube imaging can be achieved in live brain tissue using ultralow excitation doses (~0.1 kW/cm 2 ), an order of magnitude lower than those currently used. To accomplish this, we synthesized fluorescent sp 3 -defect tailored (6,5) carbon nanotubes which, when excited at their first order excitonic transition (~985 nm) fluoresce brightly at ~1160 nm. The biocompatibility of these functionalized nanotubes, which are wrapped by encapsulation agent (phospholipid-polyethylene glycol), is demonstrated using standard cytotoxicity assays. Single molecule photophysical studies of these biocompatible nanotubes allowed us to identify the optimal luminescence properties in the context of biological imaging.
dc.language.isoen
dc.publisherNature Publishing Group
dc.title.enFluorescent sp3 Defect-Tailored Carbon Nanotubes Enable NIR-II Single Particle Imaging in Live Brain Slices at Ultra-Low Excitation Doses
dc.typeArticle de revue
dc.identifier.doi10.1038/s41598-020-62201-w
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
bordeaux.journalScientific Reports
bordeaux.volume10
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.issue1
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-03409870
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03409870v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=Scientific%20Reports&amp;rft.date=2020-12&amp;rft.volume=10&amp;rft.issue=1&amp;rft.eissn=2045-2322&amp;rft.issn=2045-2322&amp;rft.au=MANDAL,%20Amit%20Kumar&amp;WU,%20Xiaojian&amp;FERREIRA,%20Joana&amp;KIM,%20Mijin&amp;POWELL,%20Lyndsey&amp;rft.genre=article


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