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A 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells (hNSC)

hal.structure.identifierUniversité de Genève = University of Geneva [UNIGE]
hal.structure.identifierLaboratoire Physico-Chimie Curie [Institut Curie] [PCC]
dc.contributor.authorALESSANDRI, Kevin
hal.structure.identifierUniversité de Genève = University of Geneva [UNIGE]
dc.contributor.authorFEYEUX, Maxime
hal.structure.identifierInstitut de Génétique et de Biologie Moléculaire et Cellulaire [IGBMC]
hal.structure.identifierLaboratoire Physico-Chimie Curie [Institut Curie] [PCC]
hal.structure.identifierBiologie Cellulaire et Cancer
dc.contributor.authorGURCHENKOV, Basile
hal.structure.identifierUniversité de Genève = University of Geneva [UNIGE]
dc.contributor.authorDELGADO, Christophe
hal.structure.identifierUniversité de Genève = University of Geneva [UNIGE]
dc.contributor.authorTRUSHKO, Anastasiya
hal.structure.identifierUniversité de Genève = University of Geneva [UNIGE]
dc.contributor.authorKRAUSE, Karl-Heinz
hal.structure.identifierBiologie Cellulaire et Cancer
dc.contributor.authorVIGNJEVIĆ, Daniela
hal.structure.identifierUniversité de Genève = University of Geneva [UNIGE]
hal.structure.identifierLaboratoire Physico-Chimie Curie [Institut Curie] [PCC]
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorNASSOY, Pierre
hal.structure.identifierUniversité de Genève = University of Geneva [UNIGE]
dc.contributor.authorROUX, Aurélien
dc.date.accessioned2023-05-12T10:32:45Z
dc.date.available2023-05-12T10:32:45Z
dc.date.issued2016
dc.identifier.issn1473-0197
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181443
dc.description.abstractEnWe present here a microfluidic device that generates sub-millimetric hollow hydrogel spheres, encapsulating cells and coated internally with a layer of reconstituted extracellular matrix (ECM) of a few microns thick. The spherical capsules, composed of alginate hydrogel, originate from the spontaneous instability of a multi-layered jet formed by co-extrusion using a coaxial flow device. We provide a simple design to manufacture this device using a DLP (digital light processing) 3D printer. Then, we demonstrate how the inner wall of the capsules can be decorated with a continuous ECM layer that is anchored to the alginate gel and mimics the basal membrane of a cellular niche. Finally, we used this approach to encapsulate human Neural Stem Cells (hNSC) derived from human Induced Pluripotent Stem Cells (hIPSC), which were further differentiated into neurons within the capsules with negligible loss of viability. Altogether, we show that these capsules may serve as cell micro-containers compatible with complex cell culture conditions and applications. These developments widen the field of research and biomedical applications of the cell encapsulation technology.
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.title.enA 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells (hNSC)
dc.typeArticle de revue
dc.identifier.doi10.1039/c6lc00133e
dc.subject.halSciences du Vivant [q-bio]/Biologie cellulaire
dc.subject.halSciences du Vivant [q-bio]/Biotechnologies
dc.subject.halSciences du Vivant [q-bio]/Ingénierie biomédicale
dc.subject.halSciences du Vivant [q-bio]/Neurosciences [q-bio.NC]
bordeaux.journalLab on a Chip
bordeaux.page1593-1604
bordeaux.volume16
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.issue9
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-03677882
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03677882v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Lab%20on%20a%20Chip&rft.date=2016&rft.volume=16&rft.issue=9&rft.spage=1593-1604&rft.epage=1593-1604&rft.eissn=1473-0197&rft.issn=1473-0197&rft.au=ALESSANDRI,%20Kevin&FEYEUX,%20Maxime&GURCHENKOV,%20Basile&DELGADO,%20Christophe&TRUSHKO,%20Anastasiya&rft.genre=article


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