Single nanotube imaging to probe complex environments
| hal.structure.identifier | lp2n-04,lp2n-12 | |
| dc.contributor.author | COGNET, Laurent | |
| dc.date.accessioned | 2023-05-12T10:27:49Z | |
| dc.date.available | 2023-05-12T10:27:49Z | |
| dc.date.conference | 2011-07-15 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/181325 | |
| dc.description.abstractEn | The optical microscopy of single molecules has recently been beneficial for many applications, in particular in biology. It allows a sub-wavelength localization of the molecules and a subtle probing of the spatio temporal nano-environment of the molecules (1). For many bio-applications, near infrared nanoprobes and/or more photostable nanoprobes than conventional fluorescent molecules or quantum dots are desirable. In this context, single walled carbon nanotube tubes (SWNTs) are promising. On the one hand, they bear excellent absorption properties which make them suitable for detection at the single particle level using the photothermal heterodyne imaging (PHI) method (2). PHI is a far-field optical method allowing the ultra-sensitive detection of tiny absorbing individual nano-objects such as gold nanoparticle down to 1.4nm, semiconductor nanocrystals or SWNTs (2). Approaches were further developed to measure the diffusion of proteins labelled with 5 nm gold nanoparticles in living cells for arbitrary long times opening the route for the detection and tracking of metallic and semiconducting SWNTs in living cells by PHI(2). On the other hand, the luminescence properties of semiconducting SWNTs are highly sensitive to the nanotubes environment. Single-molecule chemical reactions with individual SDBS wrapped nanotubes could be observed through the stepwise changes of the luminescence intensity within submicrometer segments of nanotubes (3). For further applications in biology, this sensitivity can either be useful or should be avoided by appropriate nanotube encapsulation (4). Due to their one dimensional character, understanding the thermal motion of SWNTs in crowded environments is key for applications in biology including sensing, drug delivery etc.. SWNTs behave like stiff filaments (5) and despite decades of theoretical study, the fundamental dynamics of such systems remains a mystery. Surprisingly, using highly luminescent SWNTs confined in biocompatible crowed environments (agarose networks), we find that even a small bending flexibility strongly enhances their motion: the rotational diffusion constant is proportional to the filament bending compliance and is independent of the network porosity. This study establishes definitively the reptation dynamics of stiff filaments and provides a framework to tailor the mobility of SWNTs in confined biological environments (6). | |
| dc.language.iso | en | |
| dc.title.en | Single nanotube imaging to probe complex environments | |
| dc.type | Communication dans un congrès avec actes | |
| dc.subject.hal | Physique [physics]/Physique [physics]/Optique [physics.optics] | |
| dc.subject.hal | Physique [physics]/Physique [physics]/Biophysique [physics.bio-ph] | |
| bordeaux.hal.laboratories | Laboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298 | * |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.institution | CNRS | |
| bordeaux.country | GB | |
| bordeaux.title.proceeding | CNBMT11 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-00618245 | |
| hal.version | 1 | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-00618245v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=COGNET,%20Laurent&rft.genre=proceeding |
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