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Molecular crowding creates traffic jams of kinesin motors on microtubules

hal.structure.identifierlp2n-04,lp2n-12
dc.contributor.authorLEDUC, Cecile
hal.structure.identifierDepartment of Computer Science [Dresden]
dc.contributor.authorPADBERG-GEHLE, Kathrin
hal.structure.identifierMax Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG]
dc.contributor.authorVARGA, Vladimir
hal.structure.identifierChair of Sociology, in particular of Modeling and Simulation [SOMS]
dc.contributor.authorHELBING, Dirk
hal.structure.identifierMax Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG]
dc.contributor.authorDIEZ, Stefan
hal.structure.identifierMax Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG]
dc.contributor.authorHOWARD, Jonathon
dc.date.accessioned2023-05-12T10:25:27Z
dc.date.available2023-05-12T10:25:27Z
dc.date.created2011-05-09
dc.date.issued2012-04-17
dc.identifier.issn0027-8424
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181250
dc.description.abstractEnDespite the crowdedness of the interior of cells, microtubule-based motor proteins are able to deliver cargoes rapidly and reliably throughout the cytoplasm. We hypothesize that motor proteins may be adapted to operate in crowded environments by having molecular properties that prevent them from forming traffic jams. To test this hypothesis, we reconstituted high-density traffic of purified kinesin-8 motor protein, a highly processive motor with long end-residency time, along microtubules in a total internal-reflection fluorescence microscopy assay. We found that traffic jams, characterized by an abrupt increase in the density of motors with an associated abrupt decrease in motor speed, form even in the absence of other obstructing proteins. To determine the molecular properties that lead to jamming, we altered the concentration of motors, their processivity, and their rate of dissociation from microtubule ends. Traffic jams occurred when the motor density exceeded a critical value (density-induced jams) or when motor dissociation from the microtubule ends was so slow that it resulted in a pileup (bottleneck-induced jams). Through comparison of our experimental results with theoretical models and stochastic simulations, we characterized in detail under which conditions densityand bottleneck-induced traffic jams form or do not form. Our results indicate that transport kinesins, such as kinesin-1, may be evolutionarily adapted to avoid the formation of traffic jams by moving only with moderate processivity and dissociating rapidly from microtubule ends.
dc.language.isoen
dc.publisherNational Academy of Sciences
dc.title.enMolecular crowding creates traffic jams of kinesin motors on microtubules
dc.typeArticle de revue
dc.identifier.doi10.1073/pnas.1107281109
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
bordeaux.journalProceedings of the National Academy of Sciences of the United States of America
bordeaux.page6100
bordeaux.volume109
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-00734898
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00734898v1
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