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Fluorescence imaging of single Kinesin motors on immobilized microtubules.

hal.structure.identifierMax Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG]
dc.contributor.authorKORTEN, Till
hal.structure.identifierMax Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG]
dc.contributor.authorNITZSCHE, Bert
hal.structure.identifierMax Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG]
dc.contributor.authorGELL, Chris
hal.structure.identifierMax Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG]
dc.contributor.authorRUHNOW, Felix
hal.structure.identifierlp2n-04,lp2n-12
dc.contributor.authorLEDUC, Cecile
hal.structure.identifierMax Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG]
dc.contributor.authorDIEZ, Stefan
dc.date.accessioned2023-05-12T10:24:58Z
dc.date.available2023-05-12T10:24:58Z
dc.date.created2010-06-30
dc.date.issued2011-12-07
dc.identifier.issn1064-3745
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181231
dc.description.abstractEnRecent developments in optical microscopy and nanometer tracking have greatly improved our understanding of cytoskeletal motor proteins. Using fluorescence microscopy, dynamic interactions are now routinely observed in vitro on the level of single molecules mainly using a geometry, where fluorescently labeled motors move on surface-immobilized filaments. In this chapter, we review recent methods related to single-molecule kinesin motility assays. In particular, we aim to provide practical advice on: how to set up the assays, how to acquire high-precision data from fluorescently labeled kinesin motors and attached quantum dots, and how to analyze data by nanometer tracking.
dc.language.isoen
dc.publisherHumana Press/Springer Imprint
dc.title.enFluorescence imaging of single Kinesin motors on immobilized microtubules.
dc.typeArticle de revue
dc.identifier.doi10.1007/978-1-61779-282-3_7
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
bordeaux.journalMethods in Molecular Biology
bordeaux.page121
bordeaux.volume738
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-00761060
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00761060v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Methods%20in%20Molecular%20Biology&rft.date=2011-12-07&rft.volume=738&rft.spage=121&rft.epage=121&rft.eissn=1064-3745&rft.issn=1064-3745&rft.au=KORTEN,%20Till&NITZSCHE,%20Bert&GELL,%20Chris&RUHNOW,%20Felix&LEDUC,%20Cecile&rft.genre=article


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