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hal.structure.identifierlp2n-04,lp2n-12
dc.contributor.authorLEDUC, Cecile
hal.structure.identifierlp2n-04,lp2n-12
dc.contributor.authorSI, Satyabrata
hal.structure.identifierLaboratoire d'enzymologie et biochimie structurales [LEBS]
dc.contributor.authorGAUTIER, Jérémie
hal.structure.identifierUniversity of Geneva
dc.contributor.authorSOTO-RIBEIRO, Martinho
hal.structure.identifierUniversity of Geneva
dc.contributor.authorWEHRLE-HALLER, B.
hal.structure.identifierLaboratoire d'enzymologie et biochimie structurales [LEBS]
dc.contributor.authorGAUTREAU, Alexis
dc.contributor.authorGIANNONE, Gregory
hal.structure.identifierlp2n-04,lp2n-12
dc.contributor.authorCOGNET, Laurent
hal.structure.identifierlp2n-04,lp2n-12
dc.contributor.authorLOUNIS, Brahim
dc.date.accessioned2023-05-12T10:24:40Z
dc.date.available2023-05-12T10:24:40Z
dc.date.issued2013
dc.identifier.issn1530-6984
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181222
dc.description.abstractEnSingle molecule tracking in live cells is the ultimate tool to study subcellular protein dynamics, but it is often limited by the probe size and photostability. Due to these issues, long-term tracking of proteins in confined and crowded environments, such as intracellular spaces, remains challenging. We have developed a novel optical probe consisting of 5-nm gold nanoparticles functionalized with a small fragment of camelid antibodies that recognize widely used GFPs with a very high affinity, which we call GFP-nanobodies. These small gold nanoparticles can be detected and tracked using photothermal imaging for arbitrarily long periods of time. Surface and intracellular GFP-proteins were effectively labeled even in very crowded environments such as adhesion sites and cytoskeletal structures both in vitro and in live cell cultures. These nanobody-coated gold nanoparticles are probes with unparalleled capabilities; small size, perfect photostability, high specificity, and versatility afforded by combination with the vast existing library of GFP-tagged proteins.
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.title.enA highly specific gold nanoprobe for live-cell single-molecule imaging
dc.typeArticle de revue
dc.identifier.doi10.1021/nl304561g
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
dc.identifier.arxiv1303.0985
bordeaux.journalNano Letters
bordeaux.pagexx
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-00796925
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00796925v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nano%20Letters&rft.date=2013&rft.spage=xx&rft.epage=xx&rft.eissn=1530-6984&rft.issn=1530-6984&rft.au=LEDUC,%20Cecile&SI,%20Satyabrata&GAUTIER,%20J%C3%A9r%C3%A9mie&SOTO-RIBEIRO,%20Martinho&WEHRLE-HALLER,%20B.&rft.genre=article


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