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From local to global matrix organization by fibroblasts: a 4D laser-assisted bioprinting approach

dc.rights.licenseopenen_US
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDOUILLET, Camille
dc.contributor.authorNICODEME, Marc
dc.contributor.authorHERMANT, Loïc
dc.contributor.authorBERGERON, Vanessa
dc.contributor.authorGUILLEMOT, Fabien
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFRICAIN, Jean-Christophe
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorOLIVEIRA, Hugo
dc.contributor.authorGARCIA, Mikael
dc.date.accessioned2023-04-25T13:15:50Z
dc.date.available2023-04-25T13:15:50Z
dc.date.issued2022-01
dc.identifier.issn1758-5090en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/173191
dc.description.abstractEnFibroblasts and myofibroblasts play a central role in skin homeostasis through dermal organization and maintenance. Nonetheless, the dynamic interactions between (myo)fibroblasts and the extracellular matrix (ECM) remain poorly exploited in skin repair strategies. Indeed, there is still an unmet need for soft tissue models allowing to study the spatial-temporal remodeling properties of (myo)fibroblasts. In vivo, wound healing studies in animals are limited by species specificity. In vitro, most models rely on collagen gels reorganized by randomly distributed fibroblasts. But biofabrication technologies have significantly evolved over the past ten years. High-resolution bioprinting now allows to investigate various cellular micropatterns and the emergent tissue organizations over time. In order to harness the full dynamic properties of cells and active biomaterials, it is essential to consider ‘time’ as the 4th dimension in soft tissue design. Following this 4D bioprinting approach, we aimed to develop a novel model that could replicate fibroblast dynamic remodeling in vitro. For this purpose, (myo)fibroblasts were patterned on collagen gels with laser-assisted bioprinting (LAB) to study the generated matrix deformations and reorganizations. First, distinct populations, mainly composed of fibroblasts or myofibroblasts, were established in vitro to account for the variety of fibroblastic remodeling properties. Then, LAB was used to organize both populations on collagen gels in even isotropic patterns with high resolution, high density and high viability. With maturation, bioprinted patterns of fibroblasts and myofibroblasts reorganized into dispersed or aggregated cells, respectively. Stress-release contraction assays revealed that these phenotype-specific pattern maturations were associated with distinct lattice tension states. The two populations were then patterned in anisotropic rows in order to direct the cell-generated deformations and to orient global matrix remodeling. Only maturation of anisotropic fibroblast patterns, but not myofibroblasts, resulted in collagen anisotropic reorganizations both at tissue-scale, with lattice contraction, and at microscale, with embedded microbead displacements. Following a 4D bioprinting approach, LAB patterning enabled to elicit and orient the dynamic matrix remodeling mechanisms of distinct fibroblastic populations and organizations on collagen. For future studies, this method provides a new versatile tool to investigate in vitro dermal organizations and properties, processes of remodeling in healing, and new treatment opportunities.
dc.language.isoENen_US
dc.subject.enlaser-assisted bioprinting
dc.subject.enfibroblasts
dc.subject.entissue engineering
dc.subject.en4D bioprinting
dc.subject.enin vitro model
dc.subject.encell patterning
dc.title.enFrom local to global matrix organization by fibroblasts: a 4D laser-assisted bioprinting approach
dc.title.alternativeBiofabricationen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1088/1758-5090/ac40eden_US
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologieen_US
bordeaux.journalBiofabricationen_US
bordeaux.volume14en_US
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026en_US
bordeaux.issue2en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.institutionINSERMen_US
bordeaux.institutionCHU de Bordeauxen_US
bordeaux.institutionInstitut Bergoniéen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-04081197
hal.version1
hal.date.transferred2023-04-25T13:15:52Z
hal.exporttrue
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biofabrication&rft.date=2022-01&rft.volume=14&rft.issue=2&rft.eissn=1758-5090&rft.issn=1758-5090&rft.au=DOUILLET,%20Camille&NICODEME,%20Marc&HERMANT,%20Lo%C3%AFc&BERGERON,%20Vanessa&GUILLEMOT,%20Fabien&rft.genre=article


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