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Hydrogen‑deuterium exchange mass spectrometry to study interactions and conformational changes of proteins in paints

dc.rights.licenseopenen_US
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorGALLUZZI, Francesca
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCHAIGNEPAIN, Stephane
dc.contributor.authorARSLANOGLU, Julie
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorTOKARSKI, Caroline
dc.date.accessioned2023-04-18T08:27:38Z
dc.date.available2023-04-18T08:27:38Z
dc.date.issued2022-10-01
dc.identifier.issn0301-4622en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/173044
dc.description.abstractEnLittle is known about structural alterations of proteins within the polymeric films of paints. For the first time, hydrogen‑deuterium exchange mass spectrometry (HDX-MS) was implemented to explore the conformational alterations of proteins resulting from their interaction with inorganic pigments within the early stages of the paint film formation. Intact protein analysis and bottom-up electrospray-ionisation mass spectrometry strategies combined with progressively increasing deuterium incubation times were used to compare the protein structures of the model protein hen egg-white lysozyme (HEWL) extracted from newly dried non-pigmented films and newly dried films made from a freshly made mixture of HEWL with lead white pigment (2PbCO3 Pb(OH)2). The action of other pigments was also investigated, expanding the HDX study with a global approach to paint models of HEWL mixed with zinc white (ZnO), cinnabar (HgS) and red lead (Pb3O4) pigments. The results show structural modifications of HEWL induced by the interaction with the pigment metal ions during the paint formulation after drying and prior to ageing. Both the charge distribution of HEWL proteoforms, its oxidation rate and its deuterium absorption rate, were influenced by the pigment type, providing the first insights into the correlation of pigment type/metal cation to specific chemistries related to protein stability.
dc.language.isoENen_US
dc.title.enHydrogen‑deuterium exchange mass spectrometry to study interactions and conformational changes of proteins in paints
dc.title.alternativeBiophysical Chemistryen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.bpc.2022.106861en_US
dc.subject.halChimie/Matériauxen_US
dc.description.sponsorshipEuropeHorizon 2020 Marie Skłodowska-Curie Actions Innovative Training Network (H2020 MSCA-60 ITN)en_US
bordeaux.journalBiophysical Chemistryen_US
bordeaux.volume289en_US
bordeaux.hal.laboratoriesCBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-04072491
hal.version1
hal.date.transferred2023-04-18T08:27:40Z
hal.exporttrue
dc.rights.ccPas de Licence CCen_US
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