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Neurotensin orchestrates valence assignment in the amygdala

dc.rights.licenseopenen_US
dc.contributor.authorLI, Hao
dc.contributor.authorNAMBURI, Praneeth
dc.contributor.authorOLSON, Jacob M.
dc.contributor.authorBORIO, Matilde
dc.contributor.authorLEMIEUX, Mackenzie E.
hal.structure.identifierNeurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]
dc.contributor.authorBEYELER, Anna
dc.contributor.authorCALHOON, Gwendolyn G.
dc.contributor.authorHITORA-IMAMURA, Natsuko
dc.contributor.authorCOLEY, Austin A.
dc.contributor.authorLIBSTER, Avraham
dc.contributor.authorBAL, Aneesh
dc.contributor.authorJIN, Xin
dc.contributor.authorWANG, Huan
dc.contributor.authorJIA, Caroline
dc.contributor.authorCHOUDHURY, Sourav R.
dc.contributor.authorSHI, Xi
dc.contributor.authorFELIX-ORTIZ, Ada C.
dc.contributor.authorDE LA FUENTE, Veronica
dc.contributor.authorBARTH, Vanessa P.
dc.contributor.authorKING, Hunter O.
dc.contributor.authorIZADMEHR, Ehsan M.
dc.contributor.authorREVANNA, Jasmin S.
dc.contributor.authorBATRA, Kanha
dc.contributor.authorFISCHER, Kyle B.
dc.contributor.authorKEYES, Laurel R.
dc.contributor.authorPADILLA-COREANO, Nancy
dc.contributor.authorSICILIANO, Cody A.
dc.contributor.authorMCCULLOUGH, Kenneth M.
dc.contributor.authorWICHMANN, Romy
dc.contributor.authorRESSLER, Kerry J.
dc.contributor.authorFIETE, Ila R.
dc.contributor.authorZHANG, Feng
dc.contributor.authorLI, Yulong
dc.contributor.authorTYE, Kay M.
dc.date.accessioned2023-04-07T09:42:00Z
dc.date.available2023-04-07T09:42:00Z
dc.date.created2023
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/172882
dc.description.abstractEnThe ability to associate temporally segregated information and assign positive or negative valence to environmental cues is paramount for survival. Studies have shown that different basolateral amygdala (BLA) projections are potentiated following reward or punishment learning1–7. However, we do not yet understand how valence specific information is routed to the BLA neurons with the appropriate downstream projections. Nor do we understand how to reconcile the subsecond timescales of synaptic plasticity8–11 with the longer timescales separating the predictive cues from their outcomes. Here, we demonstrate that neurotensin (NT) neurons in the paraventricular nucleus of the thalamus (PVT) projecting to the BLA (PVT-BLA:NT) mediate valence assignment by exerting concentration-dependent modulation in BLA during associative learning. We found that optogenetic activation of the PVT-BLA:NT projection promotes reward learning, while PVT-BLA projection-specific Nt gene knockout augments punishment learning. Using genetically encoded calcium and NT sensors, we further revealed that both calcium dynamics within the PVT-BLA:NT projection and NT concentrations in the BLA are enhanced after reward learning and reduced after punishment learning. Finally, we showed that CRISPR-mediated knockout of the Nt gene in the PVT-BLA pathway blunts BLA neural dynamics and attenuates the preference to active behavioral strategies to reward and punishment predictive cues. Taken together, we have identified NT as a neuropeptide that signals valence in the BLA, and showed that NT is a critical neuromodulator that orchestrates positive and negative valence assignment in amygdala neurons by extending valence-specific plasticity to behaviorally-relevant timescales.
dc.language.isoENen_US
dc.subject.enAdult
dc.subject.enAnimal cell
dc.subject.enAnimal experiment
dc.subject.enAnimal tissue
dc.subject.enArticle
dc.subject.enAssociative learning
dc.subject.enBasolateral amygdala
dc.subject.enBrain region
dc.subject.enC57BL 6 mouse
dc.subject.enCell culture
dc.subject.enCohort analysis
dc.subject.enConfocal microscopy
dc.subject.enControlled study
dc.subject.enCRISPR-CAS9 system
dc.subject.enFemale
dc.subject.enHuman
dc.subject.enIn situ hybridization
dc.subject.enIn vivo study
dc.subject.enMale
dc.subject.enNerve cell plasticity
dc.subject.enNervous system electrophysiology
dc.subject.enNeuromodulation
dc.subject.enNonhuman
dc.subject.enNts gene
dc.subject.enOpen field test
dc.subject.enOptogenetics
dc.subject.enParaventricular thalamic nucleus
dc.subject.enProtein expression
dc.subject.enProtein function
dc.subject.enSignal transduction
dc.subject.enWhole cell patch clamp
dc.subject.enAmygdala
dc.subject.enPhysiology
dc.subject.enReward
dc.subject.enBasolateral Nuclear Complex
dc.subject.enCalcium
dc.subject.enNeurotensin
dc.subject.enNeuropeptide
dc.subject.enGlutamic acid
dc.title.enNeurotensin orchestrates valence assignment in the amygdala
dc.title.alternativeNatureen_US
dc.typeDocument de travail - Pré-publicationen_US
dc.identifier.doi10.1038/s41586-022-04964-yen_US
dc.subject.halSciences du Vivant [q-bio]/Neurosciences [q-bio.NC]en_US
dc.identifier.pubmed35859170en_US
bordeaux.hal.laboratoriesNeurocentre Magendie - U1215en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINSERMen_US
bordeaux.teamCircuits Neuronaux de l'Anxiétéen_US
bordeaux.identifier.funderIDSalk Institute for Biological Studiesen_US
bordeaux.identifier.funderIDHoward Hughes Medical Instituteen_US
bordeaux.identifier.funderIDJPB Foundationen_US
bordeaux.identifier.funderIDAlfred P. Sloan Foundationen_US
bordeaux.identifier.funderIDNew York Stem Cell Foundationen_US
bordeaux.identifier.funderIDKlingenstein Third Generation Foundationen_US
bordeaux.identifier.funderIDKavli Foundationen_US
bordeaux.identifier.funderIDDolby Family Venturesen_US
bordeaux.identifier.funderIDPioneer Funden_US
bordeaux.identifier.funderIDUehara Memorial Foundationen_US
hal.identifierhal-04062021
hal.version1
hal.date.transferred2023-04-07T09:42:39Z
hal.exporttrue
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=LI,%20Hao&NAMBURI,%20Praneeth&OLSON,%20Jacob%20M.&BORIO,%20Matilde&LEMIEUX,%20Mackenzie%20E.&rft.genre=preprint


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