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dc.rights.licenseopenen_US
dc.contributor.authorGAMBINO, Giuditta
dc.contributor.authorGALLO, Daniele
hal.structure.identifierNeurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]
dc.contributor.authorCOVELO, Ana
dc.contributor.authorFERRARO, Giuseppe
dc.contributor.authorSARDO, Pierangelo
dc.contributor.authorGIGLIA, Giuseppe
dc.date.accessioned2023-03-31T09:49:50Z
dc.date.available2023-03-31T09:49:50Z
dc.date.created2022-10
dc.date.issued2022-10
dc.identifier.issn8915-849en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/172671
dc.description.abstractEnNitric oxide (NO) is a fascinating signalling molecule implicated in a plethora of biological functions, especially at the synaptic level. Exploring neurotransmission in the hippocampus could be instrumental in the individuation of putative targets for nitric-oxide mediated neuromodulation, especially in terms of the potential repercussions on fundamental processes i.e. synaptic plasticity and excitability-related phenomena. Among these targets, endovanilloid signalling constitutes an object of study since Transient Receptors Vanilloid type 1 (TRPV1) channels possess a NO-sensitive gate modulating its activation. Also, NO has been referred to as a mediator for numerous endocannabinoid effects. Notwithstanding, the linkage between TRPV1 and NO systems in neuromodulation still remains elusive. To this end, we aim at investigating the involvement of TRPV1 in nitric oxide-mediated influence on hippocampal processes. Electrophysiological whole-cell recordings in CA1 pyramidal neurons were applied to evaluate excitatory neurotransmission in rat brain slices. Indeed, miniature excitatory postsynaptic currents (mEPSCs) were analysed upon pharmacological manipulation of TRPV1 and NO signalling pathways. In detail, only the administration of the specific TRPV1 exogenous agonist – capsaicin - reduced the frequency and amplitude of mEPSC similarly to the inhibitor of neuronal nitric oxide synthase (nNOS), 7-nitroindazole (7NI). In contrast, capsazepine, TRPV1 antagonist, does not influence excitatory transmission. The combined TRPV1 activation and nNOS blockade confirm the presence of a putative common mechanism. When we administered the endovanilloid-endocannabinoid ligand, i.e. anandamide, we unveiled a potentiation of neurotransmission that was selectively reverted by 7NI. Our data suggest that nitric oxide influences TRPV1 hippocampal signalling since these channels are not constitutively active, but can be “on-demand” activated to modulate excitation in CA1 pyramidal neurons, and that this effect is linked to nitric oxide production. © 2022 Elsevier Inc.
dc.language.isoENen_US
dc.subject.enNitric oxide
dc.subject.enTRPV1
dc.subject.enmEPSC
dc.subject.enPatch-clamp
dc.subject.enCA1
dc.subject.enAnandamide
dc.title.enTRPV1 channels in nitric oxide-mediated signalling: insight on excitatory transmission in rat CA1 pyramidal neurons
dc.title.alternativeFree Radic Biol Meden_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.freeradbiomed.2022.08.025en_US
dc.subject.halSciences du Vivant [q-bio]/Neurosciences [q-bio.NC]en_US
dc.identifier.pubmed36029909en_US
dc.description.sponsorshipEuropeSocial European Funden_US
bordeaux.journalFree Radical Biology and Medicineen_US
bordeaux.page128-136en_US
bordeaux.volume191en_US
bordeaux.hal.laboratoriesNeurocentre Magendie - U1215en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINSERMen_US
bordeaux.teamEndocannabinoïdes et Neuroadaptationen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.identifier.funderIDMinistero dell'Università e della Ricercaen_US
hal.identifierhal-04053580
hal.version1
hal.date.transferred2023-03-31T09:49:57Z
hal.exporttrue
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Free%20Radical%20Biology%20and%20Medicine&rft.date=2022-10&rft.volume=191&rft.spage=128-136&rft.epage=128-136&rft.eissn=8915-849&rft.issn=8915-849&rft.au=GAMBINO,%20Giuditta&GALLO,%20Daniele&COVELO,%20Ana&FERRARO,%20Giuseppe&SARDO,%20Pierangelo&rft.genre=article


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