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Active image optimization for lattice light sheet microscopy in thick samples

dc.rights.licenseopenen_US
hal.structure.identifierBordeaux Imaging Center [BIC]
dc.contributor.authorMALIVERT, Maxime
dc.contributor.authorHARMS, Fabrice
dc.contributor.authorVEILLY, Cynthia
dc.contributor.authorLEGRAND, Jerome
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorLI, Ziqiang
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorBAYER, Emmanuelle
hal.structure.identifierBordeaux Imaging Center [BIC]
dc.contributor.authorCHOQUET, Daniel
hal.structure.identifierBordeaux Imaging Center [BIC]
dc.contributor.authorDUCROS, Mathieu
dc.date.accessioned2023-01-26T09:26:38Z
dc.date.available2023-01-26T09:26:38Z
dc.date.issued2022-12-01
dc.identifier.issn2156-7085en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/171799
dc.description.abstractEnLattice light-sheet microscopy (LLSM) is a very efficient technique for high resolution 3D imaging of dynamic phenomena in living biological samples. However, LLSM imaging remains limited in depth due to optical aberrations caused by sample-based refractive index mismatch. Here, we propose a simple and low-cost active image optimization (AIO) method to recover high resolution imaging inside thick biological samples. AIO is based on (1) a light-sheet autofocus step (AF) followed by (2) an adaptive optics image-based optimization. We determine the optimum AIO parameters to provide a fast, precise and robust aberration correction on biological samples. Finally, we demonstrate the performances of our approach on sub-micrometric structures in brain slices and plant roots.
dc.language.isoENen_US
dc.title.enActive image optimization for lattice light sheet microscopy in thick samples
dc.title.alternativeBiomed. Opt. Express, BOEen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1364/BOE.471757en_US
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaireen_US
dc.description.sponsorshipEuropeThe function of membrane tethering in plant intercellular communicationen_US
dc.description.sponsorshipEuropeAMPA Receptor Dynamic Organization and Synaptic transmission in health and diseaseen_US
bordeaux.journalBiomedical optics expressen_US
bordeaux.page6211-6228en_US
bordeaux.volume13en_US
bordeaux.hal.laboratoriesLaboratoire de Biogenèse Membranaire (LBM) - UMR 5200en_US
bordeaux.issue12en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-03957311
hal.version1
hal.date.transferred2023-01-26T09:26:49Z
hal.exporttrue
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biomedical%20optics%20express&rft.date=2022-12-01&rft.volume=13&rft.issue=12&rft.spage=6211-6228&rft.epage=6211-6228&rft.eissn=2156-7085&rft.issn=2156-7085&rft.au=MALIVERT,%20Maxime&HARMS,%20Fabrice&VEILLY,%20Cynthia&LEGRAND,%20Jerome&LI,%20Ziqiang&rft.genre=article


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