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Changes in intra-nuclear mechanics in response to DNA damaging agents revealed by time-domain Brillouin micro-spectroscopy
| dc.rights.license | open | en_US |
| dc.contributor.author | LIU, Liwang | |
| dc.contributor.author | SIMON, Marina | |
| dc.contributor.author | MUGGIOLU, Giovanna | |
| dc.contributor.author | VILOTTE, Florent | |
| dc.contributor.author | ANTOINE, Mikael | |
| dc.contributor.author | CARON, Jerôme | |
| dc.contributor.author | KANTOR, Guy | |
| dc.contributor.author | BARBERET, Philippe
IDREF: 078137748 | |
| dc.contributor.author | SEZNEC, Hervé | |
| dc.contributor.author | AUDOIN, Bertrand
IDREF: 077252918 | |
| dc.date.accessioned | 2022-11-17T12:43:54Z | |
| dc.date.available | 2022-11-17T12:43:54Z | |
| dc.date.issued | 2022-07-11 | |
| dc.identifier.issn | 2213-5979 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/170311 | |
| dc.description.abstractEn | How DNA damage and repair processes affect the biomechanical properties of the nucleus interior remains unknown. Here, an opto-acoustic microscope based on time-domain Brillouin spectroscopy (TDBS) was used to investigate the induced regulation of intra-nuclear mechanics. With this ultrafast pump-probe technique, coherent acoustic phonons were tracked along their propagation in the intra-nucleus nanostructure and the complex stiffness moduli and thicknesses were measured with an optical resolution. Osteosarcoma cells were exposed to methyl methanesulfonate (MMS) and the presence of DNA damage was tested using immunodetection targeted against damage signaling proteins. TDBS revealed that the intra-nuclear storage modulus decreased significantly upon exposure to MMS, as a result of the chromatin decondensation and reorganization that favors molecular diffusion within the organelle. When the damaging agent was removed and cells incubated for 2 h in the buffer solution before fixation the intra-nuclear reorganization led to an inverse evolution of the storage modulus, the nucleus stiffened. The same tendency was measured when DNA double-strand breaks were caused by cell exposure to ionizing radiation. TDBS microscopy also revealed changes in acoustic dissipation, another mechanical probe of the intra-nucleus organization at the nano-scale, and changes in nucleus thickness during exposure to MMS and after recovery. | |
| dc.description.sponsorship | Echographie opto-acoustique de cellules individuelles ex vivo - ANR-17-CE11-0020 | en_US |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
| dc.subject.en | DNA damage/repair | |
| dc.subject.en | Chromatin condensation/decondensation | |
| dc.subject.en | Molecular clustering | |
| dc.subject.en | Nuclear mechanics | |
| dc.subject.en | Coherent phonon spectroscopy | |
| dc.subject.en | Picosecond ultrasonics | |
| dc.subject.en | Ultrafast opto-acoustics | |
| dc.title.en | Changes in intra-nuclear mechanics in response to DNA damaging agents revealed by time-domain Brillouin micro-spectroscopy | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1016/j.pacs.2022.100385 | en_US |
| dc.subject.hal | Sciences de l'ingénieur [physics]/Matériaux | en_US |
| bordeaux.journal | Photoacoustics | en_US |
| bordeaux.page | 100385 | en_US |
| bordeaux.volume | 27 | en_US |
| bordeaux.hal.laboratories | Institut de Mécanique et d’Ingénierie de Bordeaux (I2M) - UMR 5295 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | Bordeaux INP | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.institution | INRAE | en_US |
| bordeaux.institution | Arts et Métiers | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.identifier.funderID | Conseil Régional Aquitaine | en_US |
| bordeaux.import.source | hal | |
| hal.identifier | hal-03757798 | |
| hal.version | 1 | |
| hal.export | false | |
| workflow.import.source | hal | |
| dc.rights.cc | CC BY-NC-ND | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Photoacoustics&rft.date=2022-07-11&rft.volume=27&rft.spage=100385&rft.epage=100385&rft.eissn=2213-5979&rft.issn=2213-5979&rft.au=LIU,%20Liwang&SIMON,%20Marina&MUGGIOLU,%20Giovanna&VILOTTE,%20Florent&ANTOINE,%20Mikael&rft.genre=article |
