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dc.rights.licenseopenen_US
dc.contributor.authorLIU, Liwang
dc.contributor.authorSIMON, Marina
dc.contributor.authorMUGGIOLU, Giovanna
dc.contributor.authorVILOTTE, Florent
dc.contributor.authorANTOINE, Mikael
dc.contributor.authorCARON, Jerôme
dc.contributor.authorKANTOR, Guy
dc.contributor.authorBARBERET, Philippe
IDREF: 078137748
dc.contributor.authorSEZNEC, Hervé
dc.contributor.authorAUDOIN, Bertrand
IDREF: 077252918
dc.date.accessioned2022-11-17T12:43:54Z
dc.date.available2022-11-17T12:43:54Z
dc.date.issued2022-07-11
dc.identifier.issn2213-5979en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/170311
dc.description.abstractEnHow DNA damage and repair processes affect the biomechanical properties of the nucleus interior remains unknown. Here, an opto-acoustic microscope based on time-domain Brillouin spectroscopy (TDBS) was used to investigate the induced regulation of intra-nuclear mechanics. With this ultrafast pump-probe technique, coherent acoustic phonons were tracked along their propagation in the intra-nucleus nanostructure and the complex stiffness moduli and thicknesses were measured with an optical resolution. Osteosarcoma cells were exposed to methyl methanesulfonate (MMS) and the presence of DNA damage was tested using immunodetection targeted against damage signaling proteins. TDBS revealed that the intra-nuclear storage modulus decreased significantly upon exposure to MMS, as a result of the chromatin decondensation and reorganization that favors molecular diffusion within the organelle. When the damaging agent was removed and cells incubated for 2 h in the buffer solution before fixation the intra-nuclear reorganization led to an inverse evolution of the storage modulus, the nucleus stiffened. The same tendency was measured when DNA double-strand breaks were caused by cell exposure to ionizing radiation. TDBS microscopy also revealed changes in acoustic dissipation, another mechanical probe of the intra-nucleus organization at the nano-scale, and changes in nucleus thickness during exposure to MMS and after recovery.
dc.description.sponsorshipEchographie opto-acoustique de cellules individuelles ex vivo - ANR-17-CE11-0020en_US
dc.language.isoENen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subject.enDNA damage/repair
dc.subject.enChromatin condensation/decondensation
dc.subject.enMolecular clustering
dc.subject.enNuclear mechanics
dc.subject.enCoherent phonon spectroscopy
dc.subject.enPicosecond ultrasonics
dc.subject.enUltrafast opto-acoustics
dc.title.enChanges in intra-nuclear mechanics in response to DNA damaging agents revealed by time-domain Brillouin micro-spectroscopy
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.pacs.2022.100385en_US
dc.subject.halSciences de l'ingénieur [physics]/Matériauxen_US
bordeaux.journalPhotoacousticsen_US
bordeaux.page100385en_US
bordeaux.volume27en_US
bordeaux.hal.laboratoriesInstitut de Mécanique et d’Ingénierie de Bordeaux (I2M) - UMR 5295en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.institutionINRAEen_US
bordeaux.institutionArts et Métiersen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.identifier.funderIDConseil Régional Aquitaineen_US
bordeaux.import.sourcehal
hal.identifierhal-03757798
hal.version1
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccCC BY-NC-NDen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Photoacoustics&rft.date=2022-07-11&rft.volume=27&rft.spage=100385&rft.epage=100385&rft.eissn=2213-5979&rft.issn=2213-5979&rft.au=LIU,%20Liwang&SIMON,%20Marina&MUGGIOLU,%20Giovanna&VILOTTE,%20Florent&ANTOINE,%20Mikael&rft.genre=article


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