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Sequestration of Proteins by Fatty Acid Coacervates for Their Encapsulation within Vesicles

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGARENNE, David
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEVEN, Laure
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorNAVAILLES, Laurence
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorNALLET, Frédéric
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorDUFOURC, Erick J.
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDOULIEZ, Jean-Paul
dc.date.issued2016-07-22
dc.identifier.issn1433-7851
dc.description.abstractEnEncapsulating biological materials in lipid vesicles is of interest for mimicking cells; however, except in some particular cases, such processes do not occur spontaneously. Herein, we developed a simple and robust method for encapsulating proteins in fatty acid vesicles in high yields. Fatty acid based, membrane-free coacervates spontaneously sequester proteins and can reversibly form membranous vesicles upon varying the pH value, the precrowding feature in coacervates allowing for protein encapsulation withinvesicles. We then produced enzyme-enriched vesicles and show that enzymatic reactions can occur in these micrometric capsules. This work could be of interest in the field of synthetic biology for building microreactors.
dc.language.isoen
dc.publisherWiley-VCH Verlag
dc.subject.encoacervates
dc.subject.enencapsulation
dc.subject.enmicroreactors
dc.subject.enproteins : vesicles
dc.title.enSequestration of Proteins by Fatty Acid Coacervates for Their Encapsulation within Vesicles
dc.typeArticle de revue
dc.identifier.doi10.1002/anie.201607117
dc.subject.halChimie/Chimie théorique et/ou physique
bordeaux.journalAngewandte Chemie International Edition
bordeaux.pagepp. 13475-13479
bordeaux.volume55
bordeaux.issue43
bordeaux.peerReviewedoui
hal.identifierhal-01386797
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01386797v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Angewandte%20Chemie%20International%20Edition&rft.date=2016-07-22&rft.volume=55&rft.issue=43&rft.spage=pp.%2013475-13479&rft.epage=pp.%2013475-13479&rft.eissn=1433-7851&rft.issn=1433-7851&rft.au=GARENNE,%20David&BEVEN,%20Laure&NAVAILLES,%20Laurence&NALLET,%20Fr%C3%A9d%C3%A9ric&DUFOURC,%20Erick%20J.&rft.genre=article


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