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Modelling central metabolic fluxes by constraint-based optimization reveals metabolic reprogramming of developing Solanum lycopersicum (tomato) fruit.

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCOLOMBIÉ, Sophie
hal.structure.identifierInstitut de Mathématiques de Bordeaux [IMB]
dc.contributor.authorNAZARET, Christine
hal.structure.identifierUNIROUEN - UFR Santé [UNIROUEN UFR Santé]
dc.contributor.authorBÉNARD, Camille
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBIAIS, Benoît
dc.contributor.authorMENGIN, Virginie
dc.contributor.authorSOLÉ, Marion
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorFOUILLEN, Laëtitia
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDIEUAIDE-NOUBHANI, Martine
hal.structure.identifierPhysiopathologie mitochondriale
dc.contributor.authorMAZAT, Jean-Pierre
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEAUVOIT, Bertrand
hal.structure.identifier1074 Institut de Biologie Végétale Moléculaire : actions communes
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGIBON, Yves
dc.date.issued2015-01
dc.identifier.issn0960-7412
dc.description.abstractEnModelling of metabolic networks is a powerful tool to analyse the behaviour of developing plant organs, including fruits. Guided by our current understanding of heterotrophic metabolism of plant cells, a medium-scale stoichiometric model, including the balance of co-factors and energy, was constructed in order to describe metabolic shifts that occur through the nine sequential stages of Solanum lycopersicum (tomato) fruit development. The measured concentrations of the main biomass components and the accumulated metabolites in the pericarp, determined at each stage, were fitted in order to calculate, by derivation, the corresponding external fluxes. They were used as constraints to solve the model by minimizing the internal fluxes. The distribution of the calculated fluxes of central metabolism were then analysed and compared with known metabolic behaviours. For instance, the partition of the main metabolic pathways (glycolysis, pentose phosphate pathway, etc.) was relevant throughout fruit development. We also predicted a valid import of carbon and nitrogen by the fruit, as well as a consistent CO2 release. Interestingly, the energetic balance indicates that excess ATP is dissipated just before the onset of ripening, supporting the concept of the climacteric crisis. Finally, the apparent contradiction between calculated fluxes with low values compared with measured enzyme capacities suggest a complex reprogramming of the metabolic machinery during fruit development. With a powerful set of experimental data and an accurate definition of the metabolic system, this work provides important insight into the metabolic and physiological requirements of the developing tomato fruits.
dc.description.sponsorshipCentre français de phénomique végétale - ANR-11-INBS-0012
dc.language.isoen
dc.publisherWiley
dc.subject.encentral metabolism
dc.subject.enconstraint-based analysis
dc.subject.enflux balance analysis
dc.subject.enfruit metabolism
dc.subject.enmodelling
dc.subject.ensystems biology
dc.title.enModelling central metabolic fluxes by constraint-based optimization reveals metabolic reprogramming of developing Solanum lycopersicum (tomato) fruit.
dc.typeArticle de revue
dc.identifier.doi10.1111/tpj.12685
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halMathématiques [math]
bordeaux.journalPlant Journal
bordeaux.page24-39
bordeaux.volume81
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-01445680
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01445680v1
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