A systems biology study in tomato fruit reveals correlations between the ascorbate pool and genes involved in ribosome biogenesis, translation, and the heat-shock response
hal.structure.identifier | Génétique et Amélioration des Fruits et Légumes [GAFL] | |
dc.contributor.author | STEVENS, Rebecca | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | BALDET, Pierre | |
hal.structure.identifier | Génétique et Amélioration des Fruits et Légumes [GAFL] | |
dc.contributor.author | BOUCHET, Jean-Paul | |
hal.structure.identifier | Génétique et Amélioration des Fruits et Légumes [GAFL] | |
dc.contributor.author | CAUSSE, Mathilde | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | DEBORDE, Catherine | |
hal.structure.identifier | Génétique et Amélioration des Fruits et Légumes [GAFL] | |
dc.contributor.author | DESCHODT, Claire | |
hal.structure.identifier | Génétique et Amélioration des Fruits et Légumes [GAFL] | |
dc.contributor.author | FAUROBERT, Mireille | |
hal.structure.identifier | Génétique et Amélioration des Fruits et Légumes [GAFL] | |
dc.contributor.author | GARCHERY, Cecile | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | GARCIA, Virginie | |
hal.structure.identifier | Unité de recherche Plantes et Systèmes de Culture Horticoles [PSH] | |
dc.contributor.author | GAUTIER, Hélène | |
hal.structure.identifier | Sécurité et Qualité des Produits d'Origine Végétale [SQPOV] | |
dc.contributor.author | GOUBLE, Barbara | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | MAUCOURT, Mickael | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | MOING, Annick | |
hal.structure.identifier | Sécurité et Qualité des Produits d'Origine Végétale [SQPOV] | |
dc.contributor.author | PAGE, David | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | PETIT, Johann | |
hal.structure.identifier | Génétique et Amélioration des Fruits et Légumes [GAFL] | |
dc.contributor.author | POËSSEL, Jean-Luc | |
hal.structure.identifier | Génétique et Amélioration des Fruits et Légumes [GAFL] | |
dc.contributor.author | TRUFFAULT, Vincent | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | ROTHAN, Christophe | |
dc.date.issued | 2018 | |
dc.identifier.issn | 1664-462X | |
dc.description.abstractEn | Changing the balance between ascorbate, monodehydroascorbate, and dehydroascorbate in plant cells by manipulating the activity of enzymes involved in ascorbate synthesis or recycling of oxidized and reduced forms leads to multiple phenotypes. A systems biology approach including network analysis of the transcriptome, proteome and metabolites of RNAi lines for ascorbate oxidase, monodehydroascorbate reductase and galactonolactone dehydrogenase has been carried out in orange fruit pericarp of tomato (Solanum lycopersicum). The transcriptome of the RNAi ascorbate oxidase lines is inversed compared to the monodehydroascorbate reductase and galactonolactone dehydrogenase lines. Differentially expressed genes areinvolved in ribosome biogenesis and translation. This transcriptome inversion is also seen in response to different stresses in Arabidopsis. The transcriptome response is not well correlated with the proteome which, with the metabolites, are correlated to the activity of the ascorbate redox enzymes—ascorbate oxidase and monodehydroascorbate reductase. Differentially accumulated proteins include metacaspase, protein disulphideisomerase, chaperone DnaK and carbonic anhydrase and the metabolites chlorogenic acid, dehydroascorbate and alanine. The hub genes identified from the network analysis are involved in signaling, the heat-shock response and ribosome biogenesis. The results from this study therefore reveal one or several putative signals from the ascorbate pool which modify the transcriptional response and elements downstream. | |
dc.language.iso | en | |
dc.publisher | Frontiers | |
dc.rights.uri | http://creativecommons.org/licenses/by/ | |
dc.subject | activité enzymatique | |
dc.subject | métabolisme phénolique | |
dc.subject | expression des gènes | |
dc.subject | choc thermique | |
dc.subject | ascorbate | |
dc.subject | heat shock protein | |
dc.subject | transcriptome | |
dc.subject | tomate | |
dc.subject | analyse métabolomique | |
dc.subject.en | cellular signaling | |
dc.subject.en | heat-shock response | |
dc.subject.en | redox | |
dc.subject.en | ribosome biogenesis | |
dc.subject.en | translation | |
dc.subject.en | tomato (Solanum lycopersicum) | |
dc.subject.en | enzymic activity | |
dc.subject.en | tomato | |
dc.title.en | A systems biology study in tomato fruit reveals correlations between the ascorbate pool and genes involved in ribosome biogenesis, translation, and the heat-shock response | |
dc.type | Article de revue | |
dc.identifier.doi | 10.3389/fpls.2018.00137 | |
dc.subject.hal | Sciences du Vivant [q-bio] | |
bordeaux.journal | Frontiers in Plant Science | |
bordeaux.page | 16 p | |
bordeaux.volume | 9 | |
bordeaux.issue | 137 | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-01721265 | |
hal.version | 1 | |
hal.popular | non | |
hal.audience | Non spécifiée | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-01721265v1 | |
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